PMID: 7524681Nov 22, 1994Paper

Analysis of aggrecan and tenascin gene expression in mouse skeletal tissues by northern and in situ hybridization using species specific cDNA probes

Biochimica Et Biophysica Acta
V GlumoffE Vuorio

Abstract

Cartilage matrix is an interacting multicomponent system of collagen fibrils, fibril-associated small proteoglycans, and large proteoglycans and glycoproteins entrapped within the fibrillar network. In order to better understand the relationships between these different components we have constructed short cDNA clones for detection of mRNAs for two major noncollagenous macromolecules of cartilage matrix, aggrecan and tenascin. We subsequently determined their corresponding mRNA levels by Northern analysis in a panel of total RNAs isolated from several newborn mouse tissues. The expression of aggrecan was strictly restricted to cartilages while tenascin mRNA was present at variable levels in most of the tissues studied. The cDNA clones were also used to identify the cells responsible for aggrecan and tenascin production in newborn mouse tissues by in situ hybridization. With this technique aggrecan mRNA was detected in chondrocytes throughout the developing skeleton in a pattern very similar but not identical to those of type II and IX collagen mRNAs. In the newborn mouse skeleton tenascin and aggrecan mRNAs were expressed essentially in a mutually exclusive manner, tenascin transcripts being present in osteoblasts, periosteal a...Continue Reading

References

Dec 15, 1992·The Biochemical Journal·L Chandrasekaran, M L Tanzer
Jun 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Chiquet-Ehrismann
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaD Steinberg
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P VandenbergL Ala-Kokko
Jun 13, 1991·Biochimica Et Biophysica Acta·M MetsärantaE Vuorio
May 1, 1986·The Journal of Cell Biology·K L CrossinG M Edelman
Jan 1, 1989·The Journal of Cell Biology·M MendlerP Bruckner
Jan 1, 1989·Annual Review of Cell Biology·H P Erickson, M A Bourdon
Jan 1, 1986·Annual Review of Biochemistry·J R HassellV C Hascall
Oct 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M PacificiC M Chuong
Feb 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L J SandellR A Reife
Oct 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C Y LiuW W Kao
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K NakataT Kimura

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Citations

Aug 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A HiltunenE Vuorio
Apr 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·M MetsärantaE Vuorio
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S OhtaT Muramatsu
Oct 16, 2009·Genesis : the Journal of Genetics and Development·Stephen P HenryBenoit de Crombrugghe
Jan 1, 1995·Connective Tissue Research·E C EkholmR P Penttinen
Aug 17, 2010·Developmental Biology·H ThompsonI M McGonnell
Sep 4, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xin DuanLinda J Sandell
Nov 21, 1998·Microscopy Research and Technique·E J Mackie, L I Murphy
Oct 14, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·J T TaiD Shiffman
May 13, 1999·Nature Genetics·W BiB de Crombrugghe
Aug 20, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Annette J DahlyRichard J Roman
Jul 14, 2016·The Biochemical Journal·Carolyn M DancevicAlister C Ward
Nov 21, 2007·The Journal of Cell Biology·Debabrata PatraLinda J Sandell
Mar 30, 2019·British Journal of Pharmacology·James A DuncanJessica C F Kwok
Oct 13, 2006·Development·Makoto IkeyaYoshiki Sasai

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