Analysis of alphalactalbumin and betalactoglobulin from the rehydration of bovine colostrum powder using cloud point extraction and mass spectrometry.

Journal of Analytical Methods in Chemistry
Fan ZhangJinfeng Li

Abstract

Alphalactalbumin (α-La) and betalactoglobulin (β-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. The effects of different factors, including the surfactant concentration, sample volume, electrolyte, and pH were discussed. The optimized conditions for cloud point extraction of alphalactalbumin (α-La) and betalactoglobulin (β-Lg) can be concluded that the best surfactant is 1% (w/v) Triton X-114, 200 μL of sample volume, 150 mmol/L NaCl, and 6% (w/v) sucrose. After cloud point extraction, the capillary electrophoresis is used to check the efficiency of the extraction procedure. The results had been effectively confirmed by the characterization with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).

References

Jul 5, 1989·Journal of Molecular Biology·K R AcharyaD C Phillips
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·S A CostaI C Roberto
Mar 13, 2003·Biotechnology and Bioengineering·Carlota O Rangel-YaguiDaniel Blankschtein
Jan 18, 2006·Biochimica Et Biophysica Acta·Thom HuppertzAlan L Kelly
Apr 24, 2007·Analytica Chimica Acta·Aline Soriano LopesMarco Aurélio Zezzi Arruda

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Methods Mentioned

BETA
electrophoresis

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