Analysis of aluminum toxicity in Hordeum vulgare roots with an emphasis on DNA integrity and cell cycle

PloS One
Joanna JaskowiakIwona Szarejko

Abstract

Barley is one of the cereals that are most sensitive to aluminum (Al). Al in acid soils limits barley growth and development and, as a result, its productivity. The inhibition of root growth is a widely accepted indicator of Al stress. Al toxicity is affected by many factors including the culture medium, pH, Al concentration and the duration of the treatment. However, Al can act differently in different species and still Al toxicity in barley deserves study. Since the mechanism of Al toxicity is discussed we cytogenetically describe the effects of different doses of bioavailable Al on the barley nuclear genome-mitotic activity, cell cycle profile and DNA integrity. At the same time, we tested an established deep-water culture (DWC) hydroponics system and analyzed the effects of Al on the root system parameters using WinRHIZO software. We demonstrated the cytotoxic and genotoxic effect of Al in barley root cells. We showed that Al treatment significantly reduced the mitotic activity of the root tip cells and it also induced micronuclei and damaged nuclei. The DNA-damaging effect of Al was observed using the TUNEL test. We define the inhibitory influence of Al on DNA replication in barley. Analysis with the labelling and detectio...Continue Reading

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Citations

Nov 7, 2019·Frontiers in Plant Science·Miriam Szurman-ZubrzyckaIwona Szarejko
Feb 8, 2019·Journal of Environmental Health Science & Engineering·Zahra Zendehboodi
Aug 14, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sara FarokhzadehAbbas Mirsoleimani
Jun 27, 2019·International Journal of Molecular Sciences·Joanna JaskowiakIwona Szarejko
Dec 4, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Yang-Fei ZhouLin-Tong Yang
Nov 18, 2020·International Journal of Molecular Sciences·Joanna JaskowiakIwona Szarejko
Aug 3, 2021·Frontiers in Plant Science·Martina KintlováVojtěch Hudzieczek

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

EZ
FloMax
WinRHIZO
WinRHIZO Pro
GEOCHEM

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