Analysis of amino acid motif of penicillin-binding proteins 1a, 2b, and 2x in invasive Streptococcus pneumoniae nonsusceptible to penicillin isolated from pediatric patients in Casablanca, Morocco

BMC Research Notes
Idrissa DiawaraNaima Elmdaghri

Abstract

This study aimed to investigate the nature of the amino acid motifs found in PBPs of Streptococcus pneumoniae isolates in invasive diseases from pediatric patients at Casablanca, Morocco. Five penicillin-susceptible (PSSP), ten penicillin-intermediate (PISP), and fifteen penicillin-resistant S. pneumoniae (PRSP) were studied by PCR-RFLP and DNA sequencing of the pbp1a, - 2b, and - 2x genes. There were no changes in the conserved motifs of PBP1a, PBP2b and PBP2x for PSSP strains. Substitution close to PBP1a conserved motifs were found in all PRSP isolates and six/five PISP. Analysis of PBP2b showed that all but one of the 10 PISP strains and all PRSP had substitutions. Substitution close to PBP2x motifs showed that all but three of the 10 PISP strains and all PRSP had substitutions in tow conserved motifs. A total of 6, 11 and 10 genotypes were found after analysis of pbp1a, pbp2b, and pbp2x, respectively. The penicillin-nonsusceptible S. pneumoniae isolated in Casablanca share most amino acid substitutions of those reported worldwide, but they occurred among pneumococci with low level resistance to b-lactams.

References

Jan 1, 1991·Annual Review of Microbiology·J M Ghuysen
Jun 13, 1998·Antimicrobial Agents and Chemotherapy·A M Smith, K P Klugman
Aug 4, 1999·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·R HakenbeckD Zähner
May 29, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Peter C Appelbaum
Sep 18, 2002·Antimicrobial Agents and Chemotherapy·Kimberly A NicholDaryl J Hoban
May 20, 2004·The Journal of Antimicrobial Chemotherapy·David C BeanJohn D Klena
May 6, 2005·The Journal of Antimicrobial Chemotherapy·Dominic GrangerMichel Roger
Nov 12, 2005·The Journal of Antimicrobial Chemotherapy·Dominic GrangerMichel Roger
Dec 22, 2005·The Journal of Antimicrobial Chemotherapy·Fabio CafiniUNKNOWN Spanish Pneumococcal Network (G03/103)
Jan 6, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohamed BenbachirBahija Zaki
Mar 15, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·X ZhouY Liu
Apr 5, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Esther Yip-Mei LiuChang-Phone Fung

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Citations

Jul 28, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rosemol VargheseBalaji Veeraraghavan

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Datasets Mentioned

BETA
M90527
X16022
X16367

Methods Mentioned

BETA
PISP

Software Mentioned

ChromasPro
Basic Local Alignment Search Tool

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