Analysis of amino acid sequence variations and immunoglobulin E-binding epitopes of German cockroach tropomyosin

Clinical and Diagnostic Laboratory Immunology
Kyoung Yong JeongTai-Soon Yong

Abstract

The allergenicities of tropomyosins from different organisms have been reported to vary. The cDNA encoding German cockroach tropomyosin (Bla g 7) was isolated, expressed, and characterized previously. In the present study, the amino acid sequence variations in German cockroach tropomyosin were analyzed in order to investigate its influence on allergenicity. We also undertook the identification of immunodominant peptides containing immunoglobulin E (IgE) epitopes which may facilitate the development of diagnostic and immunotherapeutic strategies based on the recombinant proteins. Two-dimensional gel electrophoresis and immunoblot analysis with mouse anti-recombinant German cockroach tropomyosin serum was performed to investigate the isoforms at the protein level. Reverse transcriptase PCR (RT-PCR) was applied to examine the sequence diversity. Eleven different variants of the deduced amino acid sequences were identified by RT-PCR. German cockroach tropomyosin has only minor sequence variations that did not seem to affect its allergenicity significantly. These results support the molecular basis underlying the cross-reactivities of arthropod tropomyosins. Recombinant fragments were also generated by PCR, and IgE-binding epitopes ...Continue Reading

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Citations

Aug 19, 2007·International Archives of Allergy and Immunology·Kyoung Yong JeongTai-Soon Yong
Oct 4, 2007·International Archives of Allergy and Immunology·Shigeru NakanoToshihiro Yamada
Feb 25, 2014·Current Allergy and Asthma Reports·L Karla ArrudaAnna Pomés
Mar 25, 2014·International Journal for Parasitology·Sukrit SilasDavid W Dunne
Sep 6, 2012·Allergy, Asthma & Immunology Research·Myung Hyun Sohn, Kyu-Earn Kim
Nov 4, 2009·The Korean Journal of Parasitology·Tai-Soon Yong, Kyoung Yong Jeong
Apr 4, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Samuel T MindayeJay E Slater

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