Analysis of amino acids using serially coupled columns

Journal of Separation Science
T Alvarez-SeguraM C García-Alvarez-Coque

Abstract

Single conventional columns in reversed-phase liquid chromatography are insufficient for analysing the isoindoles of primary amino acids due to their limited functionality. An interesting possibility for increasing the separation power is the combination of several columns of different nature, where the length is modified by coupling small segments. This approach may require a considerable investment to have multiple lengths for each stationary phase. However, the combination of only two columns of fixed length can be enough to resolve satisfactorily relatively complex mixtures, provided that an optimised gradient program is applied. In this work, a mixture of 19 primary amino acid isoindoles found in proteins was analysed. Four stationary phases were assayed: C18, pentafluorophenyl-C18, C4 and cyano. The mixture of isoindoles was successfully resolved in practical times using a pentafluorophenyl-C18 column coupled to a C4 column, in spite of the extremely poor performance obtained when each column is used isolatedly, independently of the length. The extreme diversity in the polarities of the isoindoles and the need of extrapolating the retention behaviour in certain regions of the solvent content domain makes the modelling of ...Continue Reading

References

Nov 1, 1990·Journal of Biochemical and Biophysical Methods·B Persson, D Eaker
Jun 28, 1996·Journal of Chromatography. B, Biomedical Applications·D Fekkes
Oct 25, 2000·Journal of Chromatography. a·A M Siouffi, R Phan-Tan-Luu
Feb 11, 2005·Journal of Chromatography. a·V Concha-HerreraM C García-Alvarez-Coque
Mar 28, 2006·Journal of Chromatography. a·J R Torres-Lapasió, M C García-Alvarez-Coque
Aug 29, 2007·Analytica Chimica Acta·M C García-Alvarez-CoqueJ J Baeza-Baeza
May 8, 2008·Journal of Separation Science·Maximilian KuehnleKlaus Albert
May 7, 2010·Journal of Chromatography. a·Uwe Dieter Neue, Hans-Joachim Kuss
Jan 1, 1988·Methods in Molecular Biology·E L Harris
Aug 21, 2012·Journal of Chromatography. a·R CelaR Rodil
Sep 14, 2013·Journal of Separation Science·Kenneth R Chalcraft, Brian E McCarry
Jun 4, 2014·Journal of Chromatography. a·C Ortiz-BolsicoM C García-Alvarez-Coque
Dec 4, 2014·Journal of Chromatography. a·C Ortiz-BolsicoM C García-Alvarez-Coque
May 9, 2016·Analytica Chimica Acta·T Alvarez-SeguraM C García-Alvarez-Coque

❮ Previous
Next ❯

Citations

May 8, 2018·Journal of Separation Science·Tamara Alvarez-SeguraMaría Celia García-Alvarez-Coque
Oct 16, 2020·Journal of Separation Science·Mimi J den UijlMaarten R van Bommel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.