Analysis of anabolic steroids by partial filling micellar electrokinetic capillary chromatography and electrospray mass spectrometry

Journal of Chromatography. a
Lotta Karina AmundsenHeli Sirén

Abstract

A partial filling micellar electrokinetic capillary chromatography (PF-MEKC) separation of six anabolic androgenic steroids (androstenedione, metandienone, fluoxymesterone, methyltestosterone, 17-epimetandienone and testosterone) is introduced. The method utilises a mixed micellar solution consisting of sodium dodecyl sulphate (SDS) and sodium taurocholate. The analytes are detected with a photodiode array detector at 247 nm wavelength. Methyltestosterone is used as internal standard. The detection limits were 39 microg/L for androstenedione, 40 microg/L for testosterone, 45 microg/L for fluoxymesterone, 45-90 microg/L for 17-epimetandienone, 59 microg/L for methyltestosterone and 90 microg/L for metandienone. Linear correlation between concentration (0.1-5.0 mg/L) and detector response was obtained with r2 of 0.994 for fluoxymesterone, 0.998 for 17-epimetandienone and 0.999 for androstenedione, metandienone and testosterone. In addition, ionisation of the investigated compounds in electrospray mass spectrometry (ESI-MS) was studied in positive ion mode. The most intense signal (100%) was the protonated molecular ion [M + H]+, except for 17-epimetandienone, which gave its strongest signal at m/z corresponding to [M - H2O + H]+....Continue Reading

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Citations

May 4, 2010·Analytical and Bioanalytical Chemistry·Leena Suntornsuk
May 13, 2010·Se pu = Chinese journal of chromatography·Lijun QiuLan Zhang
Nov 16, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ilona OlędzkaTomasz Bączek
Dec 1, 2006·Electrophoresis·Manuel Silva
Feb 17, 2005·Electrophoresis·Theron J PappasLisa A Holland
Oct 18, 2007·Electrophoresis·Claudinei A SilvaMarina F M Tavares
Mar 19, 2008·Journal of Separation Science·Lotta K AmundsenHeli Sirén
Dec 21, 2010·Electrophoresis·Sami El DeebRonald Gust
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Jun 2, 2017·Environmental Science and Pollution Research International·Heli Sirén, Samira El Fellah
Aug 11, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nouf M AlourfiMohammad S El-Shahawi

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