Analysis of angiogenesis genes from paraffin-embedded breast tumor and lymph nodes

Breast Cancer Research and Treatment
Bryan P SchneiderDavid A Flockhart

Abstract

Angiogenesis is important in tumor growth and metastasis. Germ-line polymorphisms critical to the angiogenesis pathway have been shown to confer prognostic information in multiple tumor types. These genes include vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS). We extracted DNA from 53 specimens obtained from 21 patients including a primary breast tumor, and/or a histologically involved lymph node, and/or a histologically normal lymph node. We subsequently genotyped all specimens to evaluate two polymorphisms in the eNOS gene and one polymorphism in the VEGF gene. Chromatographs were generated in 145/159 (91%) samples. When assessing all polymorphisms by site, chromatographs were generated in 42/51 (83%) samples obtained from the primary tumor and 103/108 (95%) from lymph nodes. Chromatographs were generated in 46/53 (87%) samples from the T(-786)C polymorphism in the 5'-flanking region in the eNOS gene, 49/53 (92%) when assessing the Glu298Asp polymorphism in exon 7 in the eNOS gene and 50/53 samples (94%) for the C(936)T polymorphism in the VEGF gene. There was 100% concordance between analyses from the primary tumor, uninvolved lymph node, and involved lymph node from the same case. We ...Continue Reading

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Citations

Sep 25, 2007·Breast Cancer Research and Treatment·Bryan P SchneiderDavid A Flockhart
Jun 16, 2010·The Pharmacogenomics Journal·M J SikoraJ M Rae
Nov 29, 2011·Pharmacogenomics·Torben Frøstrup Hansen, Anders Jakobsen
May 4, 2016·The Pharmacogenomics Journal·S P GampenriederR Greil
Jul 9, 2008·Biochemistry. Biokhimii︠a︡·G I Abelev, T L Eraiser
Aug 27, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bryan P SchneiderKathy D Miller

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