Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique

Kisaengch'unghak chapchi. The Korean journal of parasitology
K H JooH J Rim

Abstract

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the purity of antigens used. To solve this problem, many workers have tried to find species-specific components of antigens. The present study was performed to determine the antigenic profile of crude saline extracts of 3, 5, 8 and 12-week old P. westermani worms, which were collected from experimentally infected cats, based on SDS-PAGE and immunoblot technique. The results were as follows: 1. The SDS-PAGE showed at least 30 protein bands ranging from 229 kDa to 10 kDa molecular weight. The protein components of P. westermani changed chronologically during its developmental period. The 229 kDa band was recognized only in 12-week old worms (SEP12). 2. Analysis by ELISA showed a significant increase in antibody levels at 3 weeks in infected cats using crude saline extract antigens (SEP3, SEP5, SEP8, SEP12). 3. By EITB using SEP3 and SEP5, infected cats recognized major protein bands with molecular weight of 60, 35, 28, 25 or 21 kDa at 3-12 weeks of infection, and 3 additional antigens, 19, 13 and 10 kDa, were detected at 8-12 weeks of infections....Continue Reading

References

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Citations

Oct 23, 1997·The Korean Journal of Parasitology·Y KongS Y Cho
Dec 1, 1990·Kisaengch'unghak chapchi. The Korean journal of parasitology·D W Choi
Jun 1, 1989·Kisaengch'unghak chapchi. The Korean journal of parasitology·S H LeeJ Y Chai
Jun 1, 1993·The Korean Journal of Parasitology·T S YongI S Yeo
Mar 1, 1992·Kisaengch'unghak chapchi. The Korean journal of parasitology·H J RimJ S Lee
Sep 1, 1993·The Korean Journal of Parasitology·S I Kim, S Y Cho
Jun 1, 1992·Kisaengch'unghak chapchi. The Korean journal of parasitology·Y KongS Y Cho
Jul 17, 1998·Clinical and Diagnostic Laboratory Immunology·Y KongS Y Cho

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