PMID: 8953410Nov 1, 1996Paper

Analysis of antigens recognized by autoantibodies in herpes gestationis. Usefulness of immunoblotting using a fusion protein representing an extracellular domain of the 180 kD bullous pemphigoid antigen

Journal of Dermatological Science
H MurakamiT Hashimoto

Abstract

Herpes gestationis (HG) is a rare pregnancy-associated disease. The aim of this study was to compare various immunohistochemical and immunobiochemical techniques with respect to their diagnostic sensitivity for HG. We studied 43 HG sera; only half of these reacted with the basement membrane zone (BMZ) with both indirect immunofluorescence (IF) and complement IF of normal human skin. 81% of the sera reacted with the epidermal side of 1 M NaCl-split skin. In general, titers of anti-BMZ antibodies in HG sera were lower than those in bullous pemphigoid (BP) sera. Immunoblot analysis of human epidermal extracts showed that 51% of HG sera recognized the 180 kD BP antigen (BP180) and 26% recognized the 230 kD BP antigen (BP230). We also studied the reactivity of HG sera with fusion proteins representing either the NC16a domain of human BP180 or the C-terminal region of mouse BP230. Whereas 79% of HG sera reacted with the BP180 fusion protein, only 5% recognized the BP230 fusion protein. Our results suggest that indirect IF of 1 M NaCl-split skin and immunoblotting of a fusion protein representing the BP180 NC16a domain are more sensitive techniques for the diagnosis of HG than conventional and complement IF or immunoblotting of crude ...Continue Reading

References

Jun 1, 1973·Archives of Dermatology·G Schaumburg-LeverW F Lever
Feb 1, 1983·Journal of the American Academy of Dermatology·J K ShornickJ N Gilliam
Nov 15, 1993·The Journal of Immunology : Official Journal of the American Association of Immunologists·G J GiudiceL A Diaz

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Citations

Jun 24, 1999·Journal of Dermatological Science·D Zillikens
Nov 22, 2002·Journal of Dermatological Science·Masakazu KobayashiTakeji Nishikawa
Feb 17, 1999·The British Journal of Dermatology·S HernM M Black
Oct 28, 2006·The Journal of Investigative Dermatology·Giovanni Di ZenzoGiovanna Zambruno
Dec 28, 2007·Advances in Dermatology·Giovanni Di ZenzoLuca Borradori
May 25, 2007·Journal of Cellular and Molecular Medicine·Sidonia Mihai, Cassian Sitaru
Aug 27, 2014·The British Journal of Dermatology·N TaniT Hashimoto
Sep 20, 2011·Autoimmunity·Michael KasperkiewiczEnno Schmidt
Sep 25, 2002·BJOG : an International Journal of Obstetrics and Gynaecology·Iakov ShimanovichDetlef Zillikens

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