Analysis of antimicrobial peptides from porcine neutrophils

Journal of Microbiological Methods
Joanna Wessely-SzponderAnna Smolira

Abstract

Cationic host defence peptides are important components of innate immunity in pigs and other mammalians. Most of these peptides have a direct antimicrobial activity and they also have a broad spectrum of effects on the host immune system, which may be taken into account in the introduction of novel therapeutics. Our method permits simultaneous isolation of six antibacterial peptides, i.e. prophenin-1, prophenin-2, PR-39, and protegrins 1-3 from a porcine neutrophil crude extract and characterisation of them. Among the obtained peptides the greatest bactericidal activity expressed as MBC was seen in protegrins (10 μg/ml), whereas in the other studied peptides MBC was on the level of 20 μg/ml. Minimal inhibitory concentrations (MIC) reached 10 μg/ml for protegrins 1-3 and 20 μg/ml for prophenins, and PR-39. Within the bactericidal range all isolated peptides didn't show cytotoxicity on cell lines used in our experiment.

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Citations

Apr 24, 2014·PloS One·Edwin J A VeldhuizenHenk P Haagsman
Jan 13, 2015·Journal of the Science of Food and Agriculture·Clara S F BahMichelle A McConnell
Apr 17, 2012·Developmental and Comparative Immunology·M J EdwardsJ E Deakin
Nov 29, 2014·The Journal of Membrane Biology·Richard B Lipkin, Themis Lazaridis
Sep 22, 2015·Frontiers in Cellular and Infection Microbiology·Melania Collado-RomeroJuan J Garrido
Nov 30, 2014·Applied Biochemistry and Biotechnology·Anna Smolira, Joanna Wessely-Szponder
May 19, 2017·Tissue Engineering and Regenerative Medicine·Tomasz SzponderAnna Smolira
Sep 19, 2018·Vaccines·Roel M van HartenHenk P Haagsman
May 1, 2021·Pharmaceuticals·Gabriel S OliveiraCátia Teixeira
Jun 1, 2021·Frontiers in Veterinary Science·Shuaibing ShiChengshui Liao

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