PMID: 2785681Apr 11, 1989Paper

Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)

Nucleic Acids Research
C R NewtonA F Markham


We have improved the "polymerase chain reaction" (PCR) to permit rapid analysis of any known mutation in genomic DNA. We demonstrate a system, ARMS (Amplification Refractory Mutation System), that allows genotyping solely by inspection of reaction mixtures after agarose gel electrophoresis. The system is simple, reliable and non-isotopic. It will clearly distinguish heterozygotes at a locus from homozygotes for either allele. The system requires neither restriction enzyme digestion, allele-specific oligonucleotides as conventionally applied, nor the sequence analysis of PCR products. The basis of the invention is that unexpectedly, oligonucleotides with a mismatched 3'-residue will not function as primers in the PCR under appropriate conditions. We have analysed DNA from patients with alpha 1-antitrypsin (AAT) deficiency, from carriers of the disease and from normal individuals. Our findings are in complete agreement with allele assignments derived by direct sequencing of PCR products.


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Aug 9, 1988·Biochemistry·K R Tindall, T A Kunkel
Jul 18, 1987·Lancet·M FarrallR Williamson
Jan 11, 1988·Nucleic Acids Research·K B PetersenN Gregersen
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D R EngelkeF S Collins
Feb 1, 1988·Human Genetics·D N Cooper, H Youssoufian
Jan 29, 1988·Science·E S StofletS S Sommer
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C WongH H Kazazian
Feb 1, 1986·The Journal of Clinical Investigation·T NukiwaR G Crystal
Sep 24, 1987·Nature·F F ChehabE M Rubin
Aug 1, 1987·DNA·E H LudwigB J McCarthy
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B J ConnerR B Wallace

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