Analysis of apoptotic cell death, Bcl-2, and p53 protein expression in freshly fixed and cryopreserved ovarian tissue after exposure to warm ischemia

Fertility and Sterility
Mahmoud Rezk A HusseinTommaso Falcone

Abstract

To evaluate the effect of ischemia time on the expression of Bcl-2 and p53 proteins on freshly fixed and cryopreserved-thawed ovarian tissue. Experimental study using porcine animal model. Biological Resources Unit, Cleveland Clinic Foundation. Eight nonpregnant adult sows. Bilateral oophorectomy was performed in eight sows and the ovaries were subjected to time-dependent (1, 10, 20, and 30 min) warm ischemia (room temperature). Each specimen was divided into two parts, One was fixed as a fresh tissue (freshly fixed) and the other was subjected to cryopreservation, thawing, and fixation (cryopreserved). Apoptosis (TUNEL assay) and Bcl-2 and p53 protein expression (immunoperoxidase method) were assessed. At 1, 10, 20, 30 min of warm ischemia the apoptotic indices were: 1) statistically significantly higher in the atretic (1.36 +/- 0.20, 1.59 +/- 0.20, 1.67 +/- 0.22, and 1.67 +/- 0.24, respectively) than in the nonatretic follicles (0.69 +/- 0.06, 0.69 +/- 0.06, 0.76 +/-, and 0.06, 0.71 +/- 0.06, respectively; P<.05); 2) not significantly different between freshly fixed and cryopreserved tissues; and 3) nonsignificantly higher with the increased duration of ischemia. Bcl-2 expression was seen in the granulosa but not in the theca...Continue Reading

Citations

Feb 4, 2009·Urological Research·Omer BayrakDogan Unal
Dec 11, 2008·Functional & Integrative Genomics·Alexander V SirotkinMilos Mlyncek
Oct 13, 2006·Clinical and Experimental Immunology·N A FathiN A Afifi
Apr 20, 2011·The Journal of Obstetrics and Gynaecology Research·Hye Jin ChangSeok Hyun Kim
Jul 24, 2014·Journal of Tissue Engineering and Regenerative Medicine·Bong-Wook ParkGyu-Jin Rho
Jun 29, 2014·Research in Veterinary Science·A A CarvalhoA P R Rodrigues
Jun 14, 2013·Biology of Reproduction·Deborah M Magalhães-PadilhaEduardo L Gastal
Mar 17, 2017·Zygote : the Biology of Gametes and Early Embryos·Ellen de Vasconcelos da CunhaJosé Roberto Viana Silva
Mar 1, 2009·Biopreservation and Biobanking·John M BaustJohn G Baust
Oct 28, 2017·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Riqiang BaoChunping Zhang
Aug 16, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·A V SirotkinF Valenzuela
Jun 7, 2007·Molecular Reproduction and Development·Chang Joo LeeYong-Dal Yoon
Jan 5, 2010·Organogenesis·John G BaustJohn M Baust
Sep 27, 2006·Human Reproduction·Mohamed A BedaiwyTommaso Falcone
Oct 2, 2019·Molecular Reproduction and Development·Ghassan M A IshakEduardo L Gastal
Nov 27, 2009·Systems Biology in Reproductive Medicine·Raffaella DepaloCatia Lippolis

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