Analysis of apparent co-operativity in the catecholamine-stimulated lipolysis of rat adipose tissue

Biochemical Pharmacology
J I DaviesD Everett


(1) The methods available for assessment of the regulatory features of dose-response relationships that do not conform to the Hill equation are considered, and the sensitisation index Si introduced (2) The value of determining Si is assessed using lipolytic dose-response relationships yielded by beta-adrenergic agonists both alone and in the presence of their specific antagonists [D. M. F. Cooper and J. I Davies, Biochem. Pharmac. 31, 721 (1982)]. (3) It is shown that where the Hill equation is fitted to relationships between L-noradrenaline concentration and lipolysis, the high value obtained for the Hill coefficient (1.69) is artefactual (4) By fitting a rational quadratic model and evaluating Si, a solution is obtained (Si(max), 1.02) which is free of this artefact. (5) The relationship between the results obtained by analysis of dose-response curves and direct binding of catecholamine analogues to membrane receptors is discussed.

Related Concepts

Fat Pad
Dose-Response Relationship, Drug
Norepinephrine, (+, -)-Isomer
Rats, Laboratory
In Vitro [Publication Type]

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