Analysis of Arabidopsis arginase gene transcription patterns indicates specific biological functions for recently diverged paralogs.

Plant Molecular Biology
Disa M BrownfieldMichael K Deyholos

Abstract

The detailed expression patterns of transcripts of two Arabidopsis arginase genes, ARGAH1 and ARGAH2, have not been previously described, and phylogenetic analysis suggests that they diverged independently of duplication events in other lineages. Therefore, we used beta-glucuronidase reporter fusions and quantitative reverse-transcriptase PCR to analyze tissue-specific expression of ARGAH1 and ARGAH2 during Arabidopsis development, and in response to the availability of nutrients and exposure to methyl jasmonate (MeJA). We demonstrated tissue-specific transcript expression and enzyme activity in pollen for ARGAH1, but not ARGAH2. Conversely, we demonstrated MeJA-inducibility of ARGAH2, but not ARGAH1. In addition, we used microarrays to identify genes for which transcript abundance following MeJA treatment differed in wild type and ARGAH2 mutants. These ARGAH2 and MeJA responsive genes included a putative pathogenesis-related protein pathogenesis response-1 (At2g14610), and a gene of unknown function (At5g03090). Interestingly, these genes had opposite responses to the loss of ARGAH2, suggesting multiple downstream effects of arginase activity, following MeJA treatment. These results, and the variety and complexity of expressio...Continue Reading

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Citations

Mar 22, 2012·Plant & Cell Physiology·Antoine GravotMaria J Manzanares-Dauleux
Dec 26, 2012·Plant Science : an International Journal of Experimental Plant Biology·Joe C PolaccoTiago Tezotto
Jul 16, 2010·Journal of the Science of Food and Agriculture·Xinhua ZhangJiping Sheng
Aug 19, 2015·Frontiers in Plant Science·Gudrun WinterDietmar Funck
Apr 24, 2017·Environmental Science and Pollution Research International·Aziz KhanShah Fahad

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