Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules.

Plant Physiology
Martina B ZellVerónica G Maurino

Abstract

While malate and fumarate participate in a multiplicity of pathways in plant metabolism, the function of these organic acids as carbon stores in C(3) plants has not been deeply addressed. Here, Arabidopsis (Arabidopsis thaliana) plants overexpressing a maize (Zea mays) plastidic NADP-malic enzyme (MEm plants) were used to analyze the consequences of sustained low malate and fumarate levels on the physiology of this C(3) plant. When grown in short days (sd), MEm plants developed a pale-green phenotype with decreased biomass and increased specific leaf area, with thin leaves having lower photosynthetic performance. These features were absent in plants growing in long days. The analysis of metabolite levels of rosettes from transgenic plants indicated similar disturbances in both sd and long days, with very low levels of malate and fumarate. Determinations of the respiratory quotient by the end of the night indicated a shift from carbohydrates to organic acids as the main substrates for respiration in the wild type, while MEm plants use more reduced compounds, like fatty acids and proteins, to fuel respiration. It is concluded that the alterations observed in sd MEm plants are a consequence of impairment in the supply of carbon sk...Continue Reading

References

Nov 15, 1978·Biochimica Et Biophysica Acta·M StittT ap Rees
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Maarten KoornneefWim Soppe
Nov 1, 1981·Plant Physiology·D K Stumpf, R H Burris
May 23, 2006·Journal of Experimental Botany·Graham NoctorBertrand Gakière
Dec 8, 2006·The Biochemical Journal·Samuel C ZeemanAlison M Smith
May 17, 2007·The Review of Scientific Instruments·I HorcasA M Baro
Jun 6, 2008·The Journal of Physical Chemistry. B·Frank SchleifenbaumAlfred J Meixner
May 29, 2009·Phytochemistry·Alisdair R Fernie, Enrico Martinoia

❮ Previous
Next ❯

Citations

Aug 21, 2012·Journal of Agricultural and Food Chemistry·Morten R ClausenHanne C Bertram
Dec 18, 2013·Journal of the Royal Society, Interface·Daniel D SeatonAlexandra Pokhilko
Sep 29, 2011·Plant Physiology·Takayuki TohgeAlisdair R Fernie
Feb 28, 2013·The Plant Cell·Marek SzecowkaStéphanie Arrivault
Dec 12, 2012·Plant Physiology and Biochemistry : PPB·Mariana SaigoMaría F Drincovich
Mar 29, 2011·Phytochemistry·Wagner L AraújoAlisdair R Fernie
Mar 8, 2016·Plant Physiology and Biochemistry : PPB·Alexander T EprintsevAbir U Igamberdiev
May 26, 2012·The New Phytologist·Kevin L Griffin, Matthew H Turnbull
Feb 22, 2011·Plant, Cell & Environment·Nima YazdanbakhshJoachim Fisahn
Sep 19, 2015·The Arabidopsis Book·Veronica G Maurino, Martin K M Engqvist
Sep 15, 2011·Molecular Plant·Klaus HarterFrank Schleifenbaum
Jul 30, 2014·Frontiers in Plant Science·Sébastien PeterFrank E Schleifenbaum
Oct 25, 2016·Plant, Cell & Environment·Imke I HoermillerArnd G Heyer
Nov 9, 2016·Frontiers in Plant Science·Maria F DrincovichVeronica G Maurino
Nov 6, 2018·The New Phytologist·Brendan M O'LearyOwen K Atkin
Apr 19, 2019·Journal of Experimental Botany·Helena A HerrmannGiles N Johnson
May 18, 2020·The New Phytologist·Fatimah Azzahra Ahmad RashidOwen K Atkin
Jun 27, 2019·Nature Plants·Clarisa E AlvarezVeronica G Maurino
Sep 5, 2019·NPJ Systems Biology and Applications·Helena A HerrmannJean-Marc Schwartz
Dec 14, 2018·Frontiers in Plant Science·Willian Batista-SilvaWagner L Araújo
Oct 28, 2017·Plant Methods·Frederik DethloffJoachim Kopka
Jan 11, 2018·Scientific Reports·Jens SchwachtjeJoachim Kopka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.