Analysis of autoantibody reactivity and common idiotype PR4 expression of myeloma proteins

Journal of Autoimmunity
R WattsD A Isenberg

Abstract

Sera from 75 patients with monoclonal gammopathies and with no clinical evidence of autoimmune disease have been screened for a wide range of autoreactivity including binding to DNA, cardiolipin, extractable nuclear antigen (ENA), rheumatoid factor activity and the presence of the common anti-DNA antibody idiotype PR4. The sera of 17/75 (23%) patients possessed autoreactivity: six were positive for anti-DNA activity, two had anticardiolipin activity and the PR4 ID was found in two sera (both of which possessed anti-DNA activity). Antibodies to ENA were found in one serum (anti-Ro) and anti-organ-specific antibodies in five. Using iso-electric focusing and immunoblotting we have shown that the PR4 ID and DNA binding activity are carried on the paraprotein and not on some other serum constituent. The IgG subclass distribution of 55 IgG paraproteins has also been investigated. The majority of IgG paraproteins belong to IgG1 subclass (55%), with the others, being IgG2 (4%), IgG3 (9%) and IgG4 (27%). In this study we have shown that sera from myeloma patients frequently possess autoreactivity, and that in many cases this can be attributed to the paraprotein.

References

Sep 1, 1988·Arthritis and Rheumatism·W WilliamsD A Isenberg
Dec 1, 1988·British Journal of Rheumatology·D A IsenbergY Shoenfeld
Feb 27, 1986·Journal of Immunological Methods·J G Shields, M W Turner
Jul 1, 1987·The Journal of Clinical Investigation·A L SestakM Reichlin
Jun 1, 1987·Annals of the Rheumatic Diseases·D A IsenbergA Todd-Pokropek
Dec 1, 1984·European Journal of Immunology·M ZoualiA Eyquem
Oct 1, 1984·Annals of the Rheumatic Diseases·I TeitssonH Valdimarsson

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Citations

Dec 1, 1990·Annals of the Rheumatic Diseases·R A Watts, D A Isenberg
Nov 1, 1992·Annals of the Rheumatic Diseases·M R Ehrenstein, D A Isenberg
Apr 1, 1994·The Journal of Clinical Investigation·M R EhrensteinD A Isenberg
Sep 1, 1995·Kidney International·M R EhrensteinD A Isenberg
Jan 1, 1990·International Reviews of Immunology·E PaulB Diamond
Jan 1, 1990·International Reviews of Immunology·R Watts, D Isenberg
Apr 1, 1993·Clinical and Experimental Immunology·M EhrensteinD A Isenberg

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