Analysis of Bacillus globigii spores by CE

Electrophoresis
Kimberly D ChichesterChrista L Colyer

Abstract

It is imperative in today's world that harmful airborne or solution-based microbes can be detected quickly and efficiently. Bacillus globigii (Bg) spores are used as a simulant for Bacillus anthracis (Ba) due to their similar shape, size, and cellular makeup. The utility of CE to separate and detect low levels of Bg spore concentrations will be evaluated. To differentiate spores from background particulates, several dyes, including fluorescamine, C-10, NN-127, Red-1c, and indocyanine green (ICG), were utilized as noncovalent labels for proteins on the Bg spore surface, as well as for HSA and homoserine standards. On-column labeling, with dye present in the running buffer, was utilized to obtain greater sensitivity and better separation. CE with LIF detection enables interactions between the dye and spore surface proteins to be observed, with enhanced fluorescence occurring upon binding of the dye to surface protein. Resulting electropherograms showed unique fingerprints for each dye with Bg spores. Migration times were under 10 min for all dye-spore complexes, with net mobilities ranging from 3.5x10(-4) to 6.9x10(-4) cm(2) V(-1) s(-1), and calibration curves yielded correlation coefficients of 0.98 or better for four of the dye...Continue Reading

References

Jun 1, 1975·Archives of Biochemistry and Biophysics·A M FelixM Weigele
Mar 1, 1973·Archives of Biochemistry and Biophysics·S SteinS Udenfriend
Jul 1, 1974·Archives of Biochemistry and Biophysics·S De BernardoS Udenfriend
Jul 1, 1995·Annals of Biomedical Engineering·P R van den BiesenD W Slaaf
May 1, 1995·AJR. American Journal of Roentgenology·D L BerridgeD J Czarnecki
Nov 1, 1993·Bio/technology·R C Ebersole, R M McCormick
Jun 20, 1998·Journal of Applied Microbiology·R G Clarke, A C Pinder
Aug 27, 1998·Journal of Chromatography. a·R Zhu, W T Kok
Oct 3, 1999·Applied and Environmental Microbiology·Y HathoutC Fenselau
Jan 7, 2000·Analytical Chemistry·D W ArmstrongD J Westenberg
Jul 29, 2000·FEMS Microbiology Letters·J M SchneiderheinzeD J Westenberg
Aug 17, 2000·Biosensors & Bioelectronics·G P AndersonL H Johnson
Sep 6, 2000·Letters in Applied Microbiology·P Longchamp, T Leighton
Dec 29, 2000·Analytical Chemistry·D R Walt, D R Franz
Jan 26, 2002·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Leila TaraziLucjan Strekowski
Jan 30, 2003·Analytical Chemistry·Dimitra N Stratis-CullumTuan Vo-Dinh
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Frank WelderChrista L Colyer
Dec 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael A Rodriguez, Daniel W Armstrong
May 29, 2004·Analytical Chemistry·B PalenzuelaM Valcárcel
Jan 15, 2005·Analytical Chemistry·Svetlana FarrellWilliam R Heineman
Mar 3, 2005·Biosensors & Bioelectronics·Pierre N FlorianoJohn T McDevitt
Jul 28, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Zhongmin XiangSteven A Reeves
Aug 24, 2005·Journal of Chromatography. a·Michał SzumskiBogusław Buszewski
Jul 13, 2006·Journal of Separation Science·Ewa KłodzińiskaBogusław Buszewski
May 16, 2005·Optics Express·P KayeS J Barrington

❮ Previous
Next ❯

Citations

Apr 24, 2009·Analytical Chemistry·Marie HorkáKarel Slais
May 26, 2010·Analytical Chemistry·Nicholas W FrostMichael T Bowser
Jul 10, 2021·The Journal of the Acoustical Society of America·Filip PlazonicPeter Glynne-Jones

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.