Analysis of bacterial adhesion using a gradient force analysis method and colloid probe atomic force microscopy

Langmuir : the ACS Journal of Surfaces and Colloids
Xu Li, Bruce E Logan

Abstract

The atomic force microscope (AFM) has been used to examine the stickiness of bacteria on the basis of the analysis of approach and retraction force curves between the AFM tip and the bacterial surface. One difficulty in analyzing approach curve data is that the distance between the AFM tip and the surface of the bacterium is difficult to define. The exact distances are difficult to determine because the surface of the bacterium deforms during force imaging, producing a highly nonlinear region in the approach curve. In this study, AFM approach and retraction curves were obtained using a colloid probe AFM for three strains of Escherichia coli (D21, D21f2, and JM109). These strains differed in their relative adhesion to glass surfaces, on the basis of measurements of sticking coefficients in packed bed flow through column tests. A gradient force curve analysis method was developed to model the interactions between the colloid probe and a surface. Gradient analysis of the approach curve revealed four different regions of colloid-surface interactions during the approach and contact of the probe with the bacterial surface: a noninteraction region, a noncontact phase, a contact phase, and a constant compliance region. The noncontact p...Continue Reading

References

Jun 6, 2003·Environmental Science & Technology·Nehal I Abu-Lail, Terri A Camesano
Apr 10, 2004·Microbiology·Virginia Vadillo-RodríguezHenny C van der Mei

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Citations

Sep 17, 2013·Environmental Science & Technology·Muhammad Tariq KhanJean Philippe Croue
Oct 10, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Tugba OzdemirJustin L Brown
Dec 6, 2012·Beilstein Journal of Nanotechnology·Cagri UzümRegine von Klitzing
Feb 20, 2016·Biotechnology Letters·W R Z Wan DagangZ Zhang
Oct 30, 2012·Colloids and Surfaces. B, Biointerfaces·Peng HanMeiping Tong
May 10, 2011·Biomaterials·Elisabeth M DavisRandall T Irvin
Jun 23, 2007·Journal of Colloid and Interface Science·Beng Joo Reginald Thio, J Carson Meredith
Jan 2, 2007·Advances in Colloid and Interface Science·Daniel J JohnsonNidal Hilal
Feb 20, 2015·International Journal of Environmental Health Research·Rok FinkKlemen Bohinc
May 6, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Changjin HuangSulin Zhang
Apr 15, 2015·Biointerphases·Svetlana A ErmolaevaAlexander L Gintsburg
Feb 9, 2012·Science Progress·Aaron P Mosier, Nathaniel C Cady
Jun 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Erik RettlerUlrich S Schubert
Nov 28, 2020·Water Research·Bingdi CaoDongsheng Wang
Mar 21, 2008·Environmental Science & Technology·Zhen-Hu HuHan-Qing Yu
Sep 3, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Beng Joo Reginald ThioArturo A Keller
Dec 3, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Fatma Pinar Gordesli, Nehal I Abu-Lail
Sep 20, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Sanjai J Parikh, Jon Chorover
May 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·J J Valle-DelgadoM W Rutland

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