Analysis of bacterial and fungal communities in Japanese- and Chinese-fermented soybean pastes using nested PCR-DGGE.

Current Microbiology
Tae Woon KimHae-Yeong Kim

Abstract

The microbial diversity of Japanese- and Chinese-fermented soybean pastes was investigated using nested PCR-denaturing gradient gel electrophoresis (DGGE). Five Japanese-fermented soybean paste samples and three Chinese-fermented soybean paste samples were analyzed for bacteria and fungi. Extracted DNA was used as a template for PCR to amplify 16S rRNA and 18S rRNA genes. The nearly complete 16S rRNA and 18S rRNA genes were amplified using universal primers, and the resulting products were subsequently used as a template in a nested PCR to obtain suitable fragments for DGGE. Tetragenococcus halophilus and Staphylococcus gallinarum were found to dominate the bacterial microbiota in Japanese samples, whereas Bacillus sp. was detected as the predominant species in Chinese samples. DGGE analysis of fungi in soybean pastes determined the presence of Aspergillus oryzae and Zygosaccharomyces rouxii in most of the Chinese and Japanese samples. Some differences were observed in the bacterial diversity of Japanese- and Chinese-fermented soybean pastes.

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Apr 23, 2002·Journal of Applied Microbiology·T OndaK Yokotsuka
Dec 31, 2002·International Journal of Food Microbiology·Sara FasoliSandra Torriani
Jul 16, 2003·Letters in Applied Microbiology·T OndaK Yokotsuka
Feb 18, 2004·Journal of Microbiological Methods·Danilo Ercolini
Dec 5, 2006·Applied and Environmental Microbiology·Mitali DasLaura G Leff
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Yanchang LiangYing Lin

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Citations

Sep 14, 2013·World Journal of Microbiology & Biotechnology·Xiao-Fei DingRong-Qing Zhou
Jun 25, 2013·International Journal of Food Microbiology·Luca CocolinKalliopi Rantsiou
Sep 7, 2014·World Journal of Microbiology & Biotechnology·Liqiang ZhangRongqing Zhou
Oct 4, 2014·International Journal of Food Microbiology·Phichayaphorn AryumanApinya Assavanig
Feb 7, 2012·International Journal of Food Microbiology·Young-Do NamSeong-Il Lim
Aug 19, 2015·Environmental Science and Pollution Research International·Qingming ZhangCaixia Wang
Feb 10, 2012·Journal of the Science of Food and Agriculture·Tingtao ChenHua Wei
Jun 12, 2013·Journal of the Science of Food and Agriculture·Yeun HongHae-Yeong Kim
Dec 11, 2014·Food Science & Nutrition·Tinna Austen Ng'ong'ola-MananiTrude Wicklund
May 9, 2013·Journal of the Science of Food and Agriculture·Tingtao ChenHua Wei
Nov 18, 2014·Frontiers in Microbiology·Joanita SulaimanKok-Gan Chan
Apr 4, 2013·Applied Microbiology and Biotechnology·Noriaki MommaAkinori Shinmura
Apr 9, 2020·Frontiers in Microbiology·Qiaoqiao LuoWeibing Zhang
Nov 24, 2020·Critical Reviews in Food Science and Nutrition·Libo LiuBingcan Chen
Aug 14, 2019·International Journal of Food Microbiology·Min Kyung ParkYoung-Suk Kim
Jun 1, 2021·Journal of Food Science·Joanne G AllwoodDavid C Bean

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