Analysis of bacterial lipodepsipeptides by matrix-assisted laser desorption/ionisation time-of-flight and high-performance liquid chromatography with electrospray mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
S M MontiVincenzo Fogliano

Abstract

Strains of certain plant pathogenic bacteria, in particular several pathovars of Pseudomonas syringae, are known to produce cyclic lipodepsipeptides (LDPs) endowed with peculiar structural features and noticeable biological activities. In this study, a mass spectrometry procedure is proposed for screening LDP-producing bacterial strains and for identifying and assessing individual LDPs. After matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) screening of thirteen P. syringae strains for LDP production, the extracts from culture filtrates of eight positive strains were subjected to electrospray mass spectrometry for the identification of LDPs. Five strains were found to produce two forms of syringomycins (SR-E and SR-G) and two forms of syringopeptin 25 (SP25A and SP25B); two strains produced SR-E, SR-G and a new form of SP22; one strain produced syringotoxin (ST) and syringostatin A (SS-A) in addition to SP25A and SP25B. The yield in culture of two major LPDs: SR-G (3.2-13.8 mg x L(-1)) and SP25A (41.6-231.5 mg x L(-1)) was assessed by and high-performance liquid chromatography with electrospray mass spectrometry (HPLC/ESI-MS) in both scan and single ion monitoring (SIM) modes. Results of this investigation...Continue Reading

References

Sep 11, 1989·FEBS Letters·A SegreJ Y Takemoto
Jul 1, 1995·Bioscience, Biotechnology, and Biochemistry·A IsogaiA Suzuki
Jan 1, 1996·The Journal of Membrane Biology·A M FeiginJ G Brand

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Citations

Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Jos M RaaijmakersMaarten J D de Kock
May 25, 2002·Molecular Plant-microbe Interactions : MPMI·Vincenzo FoglianoMatteo Lorito
Nov 24, 2005·Antimicrobial Agents and Chemotherapy·Ingeborg GrgurinaJon Y Takemoto
Oct 20, 2007·Biochemical and Biophysical Research Communications·Maria Rosaria FulloneIngeborg Grgurina
Apr 24, 2010·FEMS Microbiology Reviews·Jos M RaaijmakersMarc Ongena
Aug 28, 2001·Journal of Mass Spectrometry : JMS

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