PMID: 8460166Mar 15, 1993Paper

Analysis of banded human chromosomes and in situ hybridization patterns by scanning force microscopy

Proceedings of the National Academy of Sciences of the United States of America
P RaschW M Heckl

Abstract

Scanning force microscopy was used to analyze banded human chromosomes and in situ hybridization patterns of biotinylated DNA probes. In standard human GTG-banded metaphase chromosome preparations (where GTG is G-banding with trypsin-Giemsa), chromosomal morphology and banding patterns were well preserved during the scanning procedure. The smallest identifiable features were in the range of about 100 nm and are similar to the typical structures seen by electron microscopy. In addition, in situ hybridization of human DNA probes of known chromosomal localization was used to map specific hybridization signals. Imaging of the precipitated crystals at the hybridization site clearly demonstrates the superior resolution of scanning force microscopy compared to conventional microscopy.

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Citations

Mar 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M WinfieldM J Miles
Jun 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·D M JondleE Henderson
Apr 12, 2003·Archives of Histology and Cytology·Shinichiro IwabuchiiEiichi Tamiya
Jul 1, 1994·Chromosoma·W FritzscheT M Jovin
Oct 21, 2004·Analytical Biochemistry·Masahiro MurakamiIsamu Hayata
Mar 3, 2005·Biosensors & Bioelectronics·Sung Woong HanJun Miyake
Mar 1, 1997·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·M J Allen
May 29, 2012·Micron : the International Research and Review Journal for Microscopy·Wouter Kalle, Padraig Strappe
Jul 1, 1997·Journal of Structural Biology·S ThalhammerW M Heckl
Apr 28, 2001·Annual Review of Physical Chemistry·H G Hansma
Sep 1, 1994·Cancer Genetics and Cytogenetics·T J McMasterM J Miles
Apr 29, 2008·Journal of Molecular Recognition : JMR·Orlando Argüello-Miranda, Giovanni Sáenz-Arce
Apr 1, 1996·Genome Génome / Conseil National De Recherches Canada·T J McMasterA Karp
Mar 13, 2003·European Biophysics Journal : EBJ·Robert W StarkWolfgang M Heckl
Jan 1, 1994·The American Journal of Physiology·R Lal, S A John

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