PMID: 7513800May 1, 1994Paper

Analysis of benzo[a]pyrene induced mutations by the use of Restriction-Site Mutation assays in aquatic species

Mutation Research
G A Kennerley, J M Parry

Abstract

Larval and toadlet stages of the clawed toad Xenopus laevis were exposed to benzo[a]pyrene in aquatic media. Mutations were analysed in a variety of restriction enzyme recognition sequences of the adult alpha 1-globin gene. Mutations were detected in the BsiLi recognition site (CCTGG) at early sampling times (6 h) in larval stages and at 8-days sampling time in post-metamorphosis toadlets. The predominant mutation detected was a G-->T base transversion at the 5th base of the CCTGG sequence. The data presented indicates that the Restriction-Site Mutation methodology has considerable potential for development as a technique for monitoring the genotoxic potential of water-borne toxins.

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Citations

Jun 12, 1996·Mutation Research·H SteingrimsdottirA R Lehmann
Dec 31, 1997·Mutation Research·B L Parsons, R H Heflich
Oct 29, 2015·Medical Anthropology·Jean M Langford
Jun 23, 2005·Death Studies·Tony Walter
Nov 26, 2013·Mortality·Hans Hadders
Dec 22, 2017·Death Studies·Andrew Dawson, Bryonny Goodwin-Hawkins

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