Analysis of breast milk fatty acid composition using dried milk samples

International Breastfeeding Journal
Kristina Harris JacksonW S Harris

Abstract

The effect of breast milk fatty acid (FA) composition, particularly levels of docosahexaenoic acid (DHA), on infant health outcomes is unclear. Part of the reason for this is difficulties in collecting, storing and shipping milk samples to the laboratory. Here we report the validation of a dried milk spot (DMS) system to measure FA composition to help overcome these obstacles. Milk FA were measured by gas chromatography and reported as percent of total FA; the FA of primary interest in this study were DHA and industrially produced trans FA (iTFA). Experiments were carried out using pooled milk samples from US (n = 5) and Malawian women (n = 50). Experiments compared liquid vs. DMS samples (n = 55), assessed stability of FA composition under different storage conditions (n = 5), and compared the results from two different labs using the same methods (n = 5). Both % DHA and % iTFA levels in liquid and DMS samples were strongly correlated (R(2) = 0.99 and 0.99, respectively, P < 0.0001). The % DHA in DMS samples was stable for up to four weeks at room temperature and up to three years at -80 °C; only slight deviations from the acceptable range of variability (±15 %) occurred in the 4 °C and -20 °C conditions for % DHA. The % iTFA ...Continue Reading

References

Feb 1, 1979·The American Journal of Clinical Nutrition·B Hall
Aug 1, 1980·The Journal of Nutrition·R A Gibson, G M Kneebone
Jan 10, 2006·Journal of Midwifery & Women's Health·Krista Nickerson
Jun 9, 2007·The American Journal of Clinical Nutrition·J Thomas BrennaLinda M Arterburn
Jul 28, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Craig L Jensen, Alexandre Lapillonne
Feb 11, 2012·Journal of Perinatology : Official Journal of the California Perinatal Association·M L BaackT Colaizy
May 25, 2012·The British Journal of Nutrition·Cristina CampoyRicardo Uauy
Jul 4, 2012·Nutritional Neuroscience·Daniel T JohnstonMichael N Dretsch
Nov 28, 2012·Pediatric Clinics of North America·Olivia Ballard, Ardythe L Morrow
Dec 12, 2012·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·V NobiliG Bedogni
Feb 7, 2014·The American Journal of Clinical Nutrition·Sheila M Innis
Apr 1, 2014·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Barbara SarterWilliam S Harris
Sep 16, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M WoltersW Ahrens
Oct 31, 2014·Journal of Perinatology : Official Journal of the California Perinatal Association·W S Harris, M L Baack

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Citations

Feb 6, 2017·International Breastfeeding Journal·Brian A JuberMichelle L Baack
Nov 14, 2016·Archives of Women's Mental Health·Md Jahirul IslamLisa Broidy
Nov 1, 2016·Journal of Mammary Gland Biology and Neoplasia·Michael C RudolphPaul S MacLean
Apr 9, 2021·The American Journal of Clinical Nutrition·Parul ChristianDaniel J Raiten

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Clinical Trials Mentioned

NCT01239693

Software Mentioned

GraphPad Prism
OmegaQuant Analytics
MiniTab

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