Analysis of Brevundimonas subvibrioides Developmental Signaling Systems Reveals Inconsistencies between Phenotypes and c-di-GMP Levels

Journal of Bacteriology
L. SperlingPatrick David Curtis

Abstract

The DivJ-DivK-PleC signaling system of Caulobacter crescentus is a signaling network that regulates polar development and the cell cycle. This system is conserved in related bacteria, including the sister genus Brevundimonas Previous studies had shown unexpected phenotypic differences between the C. crescentusdivK mutant and the analogous mutant of Brevundimonas subvibrioides, but further characterization was not performed. Here, phenotypic assays analyzing motility, adhesion, and pilus production (the latter characterized by a newly discovered bacteriophage) revealed that divJ and pleC mutants have phenotypes mostly similar to their C. crescentus homologs, but divK mutants maintain largely opposite phenotypes than expected. Suppressor mutations of the B. subvibrioides divK motility defect were involved in cyclic di-GMP (c-di-GMP) signaling, including the diguanylate cyclase dgcB, and cleD which is hypothesized to affect flagellar function in a c-di-GMP dependent fashion. However, the screen did not identify the diguanylate cyclase pleD Disruption of pleD in B. subvibrioides caused no change in divK or pleC phenotypes, but did reduce adhesion and increase motility of the divJ strain. Analysis of c-di-GMP levels in these strains...Continue Reading

References

Jan 1, 1981·Journal of Bacteriology·A FukudaY Okada
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S P WangB Ely
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·K C QuonG T Marczynski
Feb 11, 1999·Journal of Bacteriology·R S Janakiraman, Y V Brun
Jan 12, 2000·Journal of Bacteriology·M GoninY V Brun
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A S Lang, J T Beatty
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·C JacobsL Shapiro
Jun 22, 2002·Acta Crystallographica. Section D, Biological Crystallography·Stéphanie CabantousJean-Pierre Samama
Mar 8, 2003·Molecular Microbiology·Phillip AldridgeUrs Jenal
Sep 1, 1964·Bacteriological Reviews·J S POINDEXTER
Nov 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·J DeichW E Moerner
Feb 25, 2005·BioTechniques·Julio Levano-GarciaAna C R da Silva
May 12, 2005·Current Microbiology·María J MassimelliAngela T Lisa
Dec 1, 2006·Nature·Emanuele G BiondiMichael T Laub
Sep 11, 2007·Journal of Bacteriology·Sarah J ReisingerKathleen R Ryan
Oct 12, 2007·Journal of Cell Science·Erin D GoleyLucy Shapiro
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Patrick D Curtis, Yves V Brun
May 7, 2010·Molecular Microbiology·Xin Fang, Mark Gomelsky
Apr 20, 2012·Molecular Microbiology·Patrick D CurtisYves V Brun
May 18, 2013·Methods in Molecular Biology·Heike Burhenne, Volkhard Kaever

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