Analysis of canine herpesvirus gB, gC and gD expressed by a recombinant vaccinia virus

Archives of Virology
X XuanH Otsuka

Abstract

The genes encoding the canine herpesvirus (CHV) glycoprotein B (gB), gC and gD homologues have been reported already. However, products of these genes have not been identified yet. Previously, we have identified three CHV glycoproteins, gp 145/112, gp80 and gp47 using a panel of monoclonal antibodies (MAbs). To determine which CHV glycoprotein corresponds to gB, gC or gD, the putative genes of gB, gC, and gD of CHV were inserted into the thymidine kinase gene of vaccinia virus LC16mO strain under the control of the early-late promoter for the vaccinia virus 7.5-kilodalton polypeptide. We demonstrated here that gp145/112, gp80 and gp47 were the translation products of the CHV gB, gC and gD genes, respectively. The antigenic authenticity of recombinant gB, gC and gD were confirmed by a panel of MAbs specific for each glycoprotein produced in CHV-infected cells. Immunization of mice with these recombinants produced high titers of neutralizing antibodies against CHV. These results suggest that recombinant vaccinia viruses expressing CHV gB, gC and gD may be useful to develop a vaccine to control CHV infection.

Citations

Feb 23, 2013·The Journal of Veterinary Medical Science·Tatsunori MasataniXuenan Xuan
Oct 21, 2006·Vaccine·Julie KennerMarc Gurwith
Dec 31, 1997·The Journal of Veterinary Medical Science·K MaedaT Mikami
Feb 5, 1998·The Journal of Veterinary Medical Science·K MaedaT Mikami
Oct 20, 2000·The Journal of Veterinary Medical Science·K NakamichiH Otsuka
Dec 11, 1999·The Journal of Veterinary Medical Science·T TanakaN Suzuki
Jul 11, 2016·Biochemical and Biophysical Research Communications·Jae Hoon OhJong-Sang Park

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