Analysis of CDKN2A, CDKN2B, CDKN2C, and cyclin Ds gene status in hepatoblastoma

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
A IolasconF Della Ragione

Abstract

The status and the expression of cyclin-dependent kinase inhibitor A (CDKN2A) family genes, named CDKN2A, CDKN2B, and CDKN2C and of cyclin Ds (D1, D2, and D3) genes were investigated in 14 cases of human hepatoblastomas. These genes were selected because: 1) CDKN2A and CDKN2B are very frequently inactivated in human cancers; 2) cyclin Ds are overexpressed in several tumors and 3) CDKN2A is posttranscriptionally silenced in hepatocellular carcinomas. Structural analysis of the CDKN2A, CDKN2B, and CDKN2C genes in hepatoblastoma cases showed the absence of deletions and/or point mutations. Moreover, a detailed investigation of loss of heterozygosity at 9p21 and 1p32 (the chromosomal regions where CDKN2A genes are located) rules out the possible loss of one allele. Messenger RNA (mRNA) analysis showed that CDKN2C is expressed in all hepatoblastoma samples studied, while both CDKN2A and CDKN2B genes are not transcribed in the cancer specimens as well as in the matched normal liver tissues. Interestingly, an alternative mRNA expressed by the CDKN2A gene (beta-transcript) is detectable in 100% of the samples investigated. The analysis of cyclin D genes expression revealed that cyclin D1 is highly transcribed in normal hepatic tissue w...Continue Reading

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Citations

Sep 12, 2012·Brain Tumor Pathology·Robert StawskiPawel P Liberski
Apr 13, 2011·Hepatology International·Saira Sarfraz KhalidNilofer Qureshi
Sep 23, 2003·Cancer Genetics and Cytogenetics·Achille IolasconFulvio Della Ragione
Nov 21, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A MorettiA Iolascon
Apr 12, 2003·The Journal of Biological Chemistry·Fulvio Della RagioneVincenzo Zappia
Feb 14, 2020·International Journal of Molecular Sciences·Emanuela StamponeAdriana Borriello
Aug 8, 2020·Italian Journal of Pediatrics·Gang ShenHuixian Liu
Jan 23, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Abdulmohsen AlhejailyDavid P Lebrun
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Arend KochTorsten Pietsch
Jul 25, 2021·International Journal of Molecular Sciences·Emanuela StamponeAdriana Borriello

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