Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila.

Neural Development
Manuela M MoraruSimon G Sprecher

Abstract

In Drosophila, most neurogenetic research is carried out in vivo. Mammalian research demonstrates that primary cell culture techniques provide a powerful model to address cell autonomous and non-autonomous processes outside their endogenous environment. We developed a cell culture system in Drosophila using wildtype and genetically manipulated primary neural tissue for long-term observations. We assessed the molecular identity of distinct neural cell types by immunolabeling and genetically expressed fluorescent cell markers. We monitored mitotic activity of cell cultures derived from wildtype and tumorous larval brains. Our system provides a powerful approach to unveil developmental processes in the nervous system and to complement studies in vivo.

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Citations

Apr 20, 2013·Nature Protocols·Boris EggerSimon G Sprecher
Nov 22, 2013·PloS One·Catarina C F HomemJuergen A Knoblich
Feb 21, 2013·International Journal of Medical Sciences·Ruixue YinJiangyang Lu

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Methods Mentioned

BETA
transgenic

Software Mentioned

LAS AF
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis