Analysis of cell surface galactosyltransferase activity during mouse trophectodermal differentiation

Developmental Biology
H J HathawayB Babiarz

Abstract

The ectoplacental cone (EPC) of the Day 7.5 mouse embryo consists of a core of adhesive, proliferating trophoblast cells which transform to invasive trophoblast giant cells during implantation. Adhesive trophoblast cell types express monoclonally defined lactosaminoglycans (LAGs) at the cell surface; transformation to giant cells results in a loss of LAG cell surface expression (H. J. Hathaway and B. S. Babiarz, 1988, Cell Differ. 24, 55-66). LAGs can serve as substrates for cell surface galactosyltransferase (GalTase), providing an adhesive mechanism between a number of different cell types (B. D. Shur, 1984, Mol. Cell. Biochem. 61, 143-158). It was hypothesized that the LAGs in the EPC represented a substrate for a similar GalTase-mediated cell:cell adhesion system. Cell surface GalTase activity was demonstrated on EPC trophoblast on Day 7.5 of development by the incorporation of galactose from exogenous radiolabeled substrate. In 24- to 48-hr EPC trophoblast cultures the enzyme was localized by immunofluorescence to areas of cell:cell contact. Monolayers of differentiated trophoblast giant cells lacked this labeling pattern. The cell surface glycopeptide substrate for GalTase eluted as a single peak with an apparent molecula...Continue Reading

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Citations

Dec 6, 1989·Biochimica Et Biophysica Acta·B D Shur
Oct 31, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Pawel GrzmilWolfgang Engel
Oct 1, 1989·Current Opinion in Cell Biology·B D Shur
Sep 1, 1992·Anatomia, histologia, embryologia·M LehmannF Sinowatz
Aug 6, 2020·International Journal of Molecular Sciences·Manuel Alvarez-RodriguezHeriberto Rodriguez-Martinez

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