Analysis of cerebrospinal fluid γ-aminobutyric acid by capillary electrophoresis with laser-induced fluorescence detection

Electrophoresis
Mercedes CasadoRafael Artuch

Abstract

The measurement of γ-aminobutyric acid (GABA) is suitable for investigating various neurological disorders. In this study, a sensitive and selective method for free GABA quantification in cerebrospinal fluid (CSF) has been standardised. This method is based on CE with LIF detection using 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a derivatisating agent. The reaction conditions (NBD-F concentration, pH, temperature and reaction time) and the electrophoretic parameters (run buffer composition and pH and separation voltage) were optimised to obtain the maximum derivatisation efficiency and electrophoretic resolution. The best resolution was obtained using 200 mM sodium borate, 10 mM SDS, 8.5 mM β-CD, pH 10 and 20 kV voltage. The method was linear in the concentration range of 2.5-1000 nM with good inter- and intra-assay precision values. The effects of CSF handling on free GABA concentrations were also evaluated. Our results show that the time delay between CSF collection and freezing strongly increases the CSF GABA values. Age-related reference values were established in 55 paediatric controls. The influence of antiepileptic therapy on free CSF GABA was studied in 38 neuropaediatric patients. Significantly, higher GABA va...Continue Reading

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Citations

Jun 8, 2014·Journal of Inherited Metabolic Disease·A Garcia-Cazorla, S T Duarte
Oct 6, 2015·Electrophoresis·Véréna PoinsotFrançois Couderc
Mar 27, 2018·Developmental Medicine and Child Neurology·Elisenda Cortès-SaladelafontUNKNOWN Institut De Recerca Sant Joan De Déu Working Group
Nov 20, 2016·NMR in Biomedicine·Moyoko TomiyasuTakayuki Obata
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc
Jan 21, 2017·Analytical Chemistry·Mallikarjunarao GanesanaB Jill Venton

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