Analysis of CFB, a cytokinin-responsive gene of Arabidopsis thaliana encoding a novel F-box protein regulating sterol biosynthesis

Journal of Experimental Botany
Wolfram G BrennerThomas Schmülling

Abstract

Protein degradation by the ubiquitin-26S proteasome pathway is important for the regulation of cellular processes, but the function of most F-box proteins relevant to substrate recognition is unknown. We describe the analysis of the gene Cytokinin-induced F-box encoding (CFB, AT3G44326), identified in a meta-analysis of cytokinin-related transcriptome studies as one of the most robust cytokinin response genes. F-box domain-dependent interaction with the E3 ubiquitin ligase complex component ASK1 classifies CFB as a functional F-box protein. Apart from F-box and transmembrane domains, CFB contains no known functional domains. CFB is expressed in all plant tissues, predominantly in root tissue. A ProCFB:GFP-GUS fusion gene showed strongest expression in the lateral root cap and during lateral root formation. CFB-GFP fusion proteins were mainly localized in the nucleus and the cytosol but also at the plasma membrane. cfb mutants had no discernible phenotype, but CFB overexpressing plants showed several defects, such as a white upper inflorescence stem, similar to the hypomorphic cycloartenol synthase mutant cas1-1. Both CFB overexpressing plants and cas1-1 mutants accumulated the CAS1 substrate 2,3-oxidosqualene in the white stem ...Continue Reading

References

Feb 1, 1992·Trends in Biochemical Sciences·J Barber, B Andersson
Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Nov 25, 1988·Nucleic Acids Research·F Corpet
Jul 5, 1993·Biochimica Et Biophysica Acta·E M AroB Andersson
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Dec 28, 1999·Annual Review of Cell and Developmental Biology·R J Deshaies
May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A CiechanoverA L Schwartz
Jun 22, 2000·Yeast·V V Kushnirov
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J SmalleD V Straeten
Nov 15, 2000·Genome Research·J L HartleyM A Brasch
Jan 3, 2001·The Plant Journal : for Cell and Molecular Biology·H SakaiA Oka
Feb 24, 2001·Genome Biology·E T Kipreos, M Pagano
Jun 8, 2001·Annual Review of Biochemistry·C M Pickart
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·T WernerT Schmülling
Jun 12, 2002·Molecular Biology of the Cell·Wen-Hui ShenPascal Genschik
Jun 21, 2002·Methods in Enzymology·R Daniel Gietz, Robin A Woods
Aug 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jennifer M GagneRichard D Vierstra
Jan 10, 2003·Nucleic Acids Research·Mark L CroweMartin Trick
Jan 17, 2003·Plant Physiology·Rainer SchwackeReinhard Kunze
Mar 26, 2003·Genes & Genetic Systems·Wataru Sakamoto
Aug 13, 2003·Plant Physiology·Aaron M RashotteJoseph J Kieber
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Apr 14, 2004·The Plant Journal : for Cell and Molecular Biology·Kathrin SchrickGerd Jürgens
Aug 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Petr ObrdlikWolf B Frommer
Sep 21, 2004·Annual Review of Plant Biology·Jan Smalle, Richard D Vierstra
Sep 25, 2004·Bioscience, Biotechnology, and Biochemistry·Hisami YamadaTakeshi Mizuno
Feb 16, 2005·Plant Physiology·Joke AllemeerschMartin T R Kuiper
May 27, 2005·Nature·Nihal DharmasiriMark Estelle
May 27, 2005·Nature·Stefan Kepinski, Ottoline Leyser
Feb 21, 2006·Plant Physiology·Melissa S PischkeMichael R Sussman
Feb 1, 2007·Plant, Cell & Environment·Fei YuSteven Rodermel
Dec 1, 2007·BMC Plant Biology·Filip VandenbusscheDominique Van Der Straeten
Dec 11, 2007·The Plant Cell·Laurent LaplazeMalcolm Bennett

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Citations

Sep 16, 2020·Journal of Experimental Botany·Kjell De VrieseSteffen Vanneste

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