Analysis of changes in expression of IL-4/IL-13/STAT6 pathway and correlation with the selected clinical parameters in patients with atopic asthma.

International Journal of Immunopathology and Pharmacology
Adam AntczakEwa Brzeziańska-Lasota

Abstract

Asthma is associated with activation of interleukin-4 (IL-4)/interleukin-13 (IL-13)/signal transducer and activator of transcription factor-6(STAT6) inflammatory response via overexpression of all pathway components: IL-4, IL-13, and STAT6. To evaluate the association of IL-4, IL-13, and STAT6 expression and immunoexpression with atopic asthma development. Fifty patients with atopic asthma and 20 healthy controls were enrolled into the study. Relative gene expression was analyzed by qPCR method. Immunoexpression was assessed by ELISA method. The expression levels of IL-4, IL-13, and STAT6 were higher in patients compared to the controls, but a statistically significant difference was observed only for IL-13 (P = 0.03). In immunoexpression analysis, a statistically significant difference between patients and controls was found for IgE (P = 0.03). Significant positive correlations in the patient group were found between IL-13 gene expression and total level of serum IgE (rho = 0.230, P = 0.033), STAT6 gene/STAT6 protein and total level of serum IgE (STAT6: rho = 0.077, P = 0.038; STAT6: rho = 0.049, P = 0.042), IL-4, and STAT6 expression (rho = 0.098, P = 0.048). Any significant correlations were found between expression/immunoex...Continue Reading

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Citations

Dec 17, 2016·Immunological Investigations·P ContiKempuraj Duraisamy
Aug 14, 2019·Journal of Cardiovascular Pharmacology and Therapeutics·Kamal M KassemNour-Eddine Rhaleb
Dec 2, 2020·Nature Communications·Fatai S OladunniJordi B Torrelles
Feb 24, 2021·Journal of Biomolecular Structure & Dynamics·Sehrish NazZaheer Ul-Haq
Mar 29, 2021·Journal of Pharmaceutical and Biomedical Analysis·Xiaojuan YuJunzhi Wang

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Methods Mentioned

BETA
gene-knockout
enzyme-linked
ELISA
Assay
biopsy

Software Mentioned

Statistica
TaqMan Relative Quantification Assay

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