PMID: 2102081Dec 1, 1990Paper

Analysis of cholesterol ester accumulation in macrophages by the use of digital imaging fluorescence microscopy

Atherosclerosis
E KorenP Alaupovic

Abstract

Low density lipoprotein (LDL) induced accumulation of cholesterol esters was analyzed by the digital imaging fluorescence microscopy (DIFM) in murine tumor macrophages. To analyze cholesterol ester accumulation, P388D1 macrophages were incubated with increasing quantities of unmodified or acetylated human LDL, washed, and live stained with a lipophylic fluorescent dye Nile Red. The increase in fluorescence intensity was quantitatively determined by the interactive laser cytometer (ACAS 470) and compared with the accumulation of cellular cholesterol esters determined by the gas liquid chromatography. Correlation between the two methods was highly significant (r greater than 0.9, P less than 0.001). A good agreement between the two methods was also found in terms of sensitivity and reproducibility. With the use of 589 nm narrowband interference filter in the light path of emitted light the intensity of fluorescence correlated well with cellular cholesterol ester content even in the presence of relatively high concentrations of triglycerides. Therefore, digital imaging fluorescence microscopy appears to be a reliable method for quantification of cholesterol ester accumulation at the single cell level offering new possibilities of ...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Jan 1, 1988·Journal of Cell Science. Supplement·A M FogelmanP A Edwards
Jul 18, 1987·Lancet·M J Mitchinson, R Y Ball
Apr 1, 1987·The American Journal of Clinical Nutrition·C S Wang, D R Illingworth
Jan 1, 1986·Methods in Enzymology·L C SmithJ Bryan
Jan 1, 1985·Annals of the New York Academy of Sciences·S D FowlerP Greenspan
Mar 1, 1985·The Journal of Cell Biology·P GreenspanS D Fowler
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S D Fowler, P Greenspan
Jan 1, 1980·Journal of Receptor Research·R G AndersonM S Brown
Jan 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R E PitasR W Mahley
Jan 1, 1983·Annual Review of Biochemistry·M S Brown, J L Goldstein
Oct 1, 1983·The Journal of Cell Biology·D J McGookey, R G Anderson
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R E PitasR W Mahley

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Citations

Aug 13, 2013·Analytical and Bioanalytical Chemistry·Wei-Wen ChenTa-Chau Chang
Jan 1, 1991·Progress in Lipid Research·E KorenR D Fugate
Feb 16, 2002·In Vitro & Molecular Toxicology·M K McMillianM D Johnson
Nov 30, 2011·Journal of Atherosclerosis and Thrombosis·Giovanna NicolaouClett Erridge
May 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M KlinknerW D Kerns
Sep 27, 2019·Pharmacological Reviews·Dongdong WangAtanas G Atanasov

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