Analysis of chromatin structure in mouse preimplantation embryos by fluorescent recovery after photobleaching

Epigenetics : Official Journal of the DNA Methylation Society
Masatoshi OogaFugaku Aoki

Abstract

Zygotes are totipotent cells that have the ability to differentiate into all cell types. It is believed that this ability is lost gradually and differentiation occurs along with the progression of preimplantation development. Here, we hypothesized that the loose chromatin structure is involved in the totipotency of one-cell stage embryos and that the change from loose to tight chromatin structure is associated with the loss of totipotency. To address this hypothesis, we investigated the mobility of eGFP-tagged histone H2B (eGFP-H2B), which is an index for the looseness of chromatin, during preimplantation development based on fluorescent recovery after photobleaching (FRAP) analysis. The highest mobility of eGFP-H2B was observed in pronuclei in 1-cell stage embryos and mobility gradually decreased during preimplantation development. The decrease in mobility between the 1- and 2-cell stages depended on DNA synthesis in 2-cell stage embryos. In nuclear transferred embryos, chromatin in the pseudopronuclei loosened to a level comparable to the pronuclei in 1-cell stage embryos. These results indicated that the mobility of eGFP-H2B is negatively correlated with the degree of differentiation of preimplantation embryos. Therefore, we...Continue Reading

References

Jan 1, 1984·Australian Journal of Biological Sciences·P Quinn, A J Begley
Oct 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Majumder, M L DePamphilis
Aug 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R M Schultz
Jan 1, 1993·Methods in Enzymology·J A Lawitts, J D Biggers
Feb 27, 1997·Nature·I WilmutK H Campbell
Jul 27, 1999·Zygote : the Biology of Gametes and Early Embryos·E Aoki, R M Schultz
Feb 12, 2004·Methods in Enzymology·Hiroshi KimuraPeter R Cook
Jan 10, 2006·Developmental Cell·Eran MeshorerTom Misteli
Mar 31, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sandra B Hake, C David Allis
Aug 2, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Alexander Meissner, Rudolf Jaenisch
Apr 5, 2007·Nature Protocols·Satoshi KishigamiTeruhiko Wakayama
May 9, 2008·Cell Stem Cell·Sol EfroniEran Meshorer
Nov 10, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robert M Martin, M Cristina Cardoso
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·Alexandre Gaspar-MaiaMiguel Ramalho-Santos
Dec 28, 2010·Briefings in Functional Genomics·Adam Burton, Maria-Elena Torres-Padilla
Oct 8, 2011·Biochimica Et Biophysica Acta·Cindy E J DieterenLeo G J Nijtmans
Dec 6, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Buhe NashunFugaku Aoki
Aug 8, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Koji HayakawaKunio Shiota
May 17, 2014·Genes & Development·Ana BoškovićMaria-Elena Torres-Padilla
Aug 4, 2015·Nature Structural & Molecular Biology·Takashi IshiuchiMaria-Elena Torres-Padilla

❮ Previous
Next ❯

Citations

Nov 22, 2016·The Journal of Reproduction and Development·Ryoma Yamamoto, Fugaku Aoki
Jul 3, 2018·FEBS Letters·Satoshi FunayaFugaku Aoki
Nov 9, 2019·EMBO Reports·Manuel GuthmannMaria-Elena Torres-Padilla
Oct 17, 2019·Cold Spring Harbor Perspectives in Biology·Melanie D White, Nicolas Plachta
Jul 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ken-Ichiro AbeFugaku Aoki
Feb 23, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Maria-Elena Torres-Padilla
Jan 5, 2017·Cellular and Molecular Life Sciences : CMLS·Yuki Okada, Kosuke Yamaguchi
Jun 6, 2017·The Journal of Reproduction and Development·Satoshi Funaya, Fugaku Aoki
Sep 19, 2020·Nature Communications·Michelle M HalsteadPablo J Ross
Aug 28, 2020·Development·Marion Genet, Maria-Elena Torres-Padilla
Nov 12, 2020·Scientific Reports·Kenta SugieFugaku Aoki
Jun 13, 2021·Reproductive Biology and Endocrinology : RB&E·Shi-Meng GuoLi-Quan Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence recovery after photobleaching
transfection

Software Mentioned

LSM5 exciter

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.