Analysis of chromosome behavior in intact mammalian oocytes: monitoring the segregation of a univalent chromosome during female meiosis

Human Molecular Genetics
Patricia A HuntK Mroz

Abstract

To monitor the behavior of specific chromosomes at various stages of mammalian female meiosis, we have combined immunofluorescence staining and fluorescence in situ hybridization (FISH) on intact oocytes. We have utilized this technique to evaluate the behavior of the single X chromosome in oocytes from XO female mice, providing the first observations on segregation of an achiasmate chromosome during mammalian female meiosis and its effect on the meiotic process. As has been described in other species, we found that the univalent chromosome could either segregate as an intact chromosome to one pole or divide equationally at the first meiotic division. Our results also indicate that the presence of a univalent chromosome causes severe meiotic disruption during mammalian meiosis, affecting the alignment and segregation of other chromosomes in the complement. Despite these meiotic abnormalities, the vast majority of oocytes from XO females were able to resume and successfully complete the first meiotic division. This is in contrast to previous studies of male mice with sex chromosome abnormalities where the presence of a univalent acts to arrest meiosis at metaphase of the first meiotic division. This sex-specific difference in th...Continue Reading

Citations

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