Analysis of clock gene homologs using unifoliolates as target organs in soybean (Glycine max)

Journal of Plant Physiology
Hua LiuYongfu Fu

Abstract

Soybean is a typical short-day crop, and its photoperiodic response of flowering is critical to its yield. This phenomenon was well studied during the last century, but the molecular mechanism governing it is unknown. The clock-gene homologs, GmLCL1 and GmLCL2 (Glycine max LHY/CCA1 Like 1 and 2) and GmTOC1 (Glycine max TOC1) were cloned from Glycine max L. KN18, and their expression patterns were analyzed using a system developed in this study. We employed 8h light/16h dark and 18h light/6h dark as short- and long-day conditions, respectively, because in these conditions soybean plants had significant indexes of flowering time. We also used unifoliolates, not the whole plant as Arabidopsis, as target organs for gene expression analysis. GmLCL1 and GmLCL2 had similar circadian expression patterns and both were morning genes, while GmTOC1 was an evening gene and peaked in the evening. The expressions of GmLCL1 and GmLCL2 were obviously antiphase to that of GmTOC1.Our study provided a system that simplified the experiments without compromising the quality of data obtained and was suitable for analyzing the molecular mechanism of flowering in soybean. GmLCL1, GmLCL2 and GmTOC1 may be the components of central clock in soybean.

References

Mar 22, 2005·Plant Physiology·Valérie HechtJames L Weller
Nov 1, 1963·Plant Physiology·B H Carpenter, K C Hamner
May 1, 1971·Plant Physiology·D E BrestK C Hamner
May 23, 2006·Cell·Isabel Bäurle, Caroline Dean
May 1, 2008·Annual Review of Plant Biology·Franziska TurckGeorge Coupland

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Citations

Feb 18, 2011·Plant Cell Reports·Xintao WangYanhui Chen
Oct 31, 2012·Journal of Biosciences·Moon Young KimSuk-Ha Lee
May 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhengjun XiaKyuya Harada
Dec 28, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Santosh B SatbhaiSetsuyuki Aoki
Jan 30, 2014·PloS One·Juliana Marcolino-GomesAlexandre Nepomuceno
May 23, 2014·Plant Physiology and Biochemistry : PPB·Jianxin FuSilan Dai
Mar 8, 2013·Seminars in Cell & Developmental Biology·C Robertson McClung
Oct 29, 2015·Plant & Cell Physiology·Dong CaoFanjiang Kong
Jun 17, 2014·Journal of Integrative Plant Biology·Lim Chee LiewPrem L Bhalla
Sep 25, 2017·International Journal of Molecular Sciences·Shaomin BianXuyan Li
Jul 25, 2009·The Plant Journal : for Cell and Molecular Biology·Ryo OkadaSetsuyuki Aoki
Dec 1, 2008·Tropical Plant Biology·Anna ZdepskiTodd P Michael
Sep 25, 2017·Science China. Life Sciences·Sheng-Rui ZhangBin Liu
Nov 15, 2019·Plant, Cell & Environment·Yu WangQiguang Xie
Feb 22, 2012·Molecular Biology Reports·Xiaofang ZhongHongyu Li
May 1, 2021·International Journal of Molecular Sciences·Hazel Marie KuganAcga Cheng

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