Analysis of close stable homolog juxtaposition during meiosis in mutants of Saccharomyces cerevisiae.

Genetics
Doris Y LuiSean M Burgess

Abstract

A unique aspect of meiosis is the segregation of homologous chromosomes at the meiosis I division. The pairing of homologous chromosomes is a critical aspect of meiotic prophase I that aids proper disjunction at anaphase I. We have used a site-specific recombination assay in Saccharomyces cerevisiae to examine allelic interaction levels during meiosis in a series of mutants defective in recombination, chromatin structure, or intracellular movement. Red1, a component of the chromosome axis, and Mnd1, a chromosome-binding protein that facilitates interhomolog interaction, are critical for achieving high levels of allelic interaction. Homologous recombination factors (Sae2, Rdh54, Rad54, Rad55, Rad51, Sgs1) aid in varying degrees in promoting allelic interactions, while the Srs2 helicase appears to play no appreciable role. Ris1 (a SWI2/SNF2 related protein) and Dot1 (a histone methyltransferase) appear to play minor roles. Surprisingly, factors involved in microtubule-mediated intracellular movement (Tub3, Dhc1, and Mlp2) appear to play no appreciable role in homolog juxtaposition, unlike their counterparts in fission yeast. Taken together, these results support the notion that meiotic recombination plays a major role in the high...Continue Reading

References

Dec 1, 1990·Genome Génome / Conseil National De Recherches Canada·J Loidl
Nov 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P B Møens, R E Pearlman
Apr 1, 1974·Journal of Bacteriology·S M Kane, R Roth
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R E Esposito, M S Esposito
Nov 1, 1995·Genes & Development·B RockmillG S Roeder
Apr 8, 1994·Science·Y ChikashigeY Hiraoka
Jun 1, 1994·The Journal of Cell Biology·J LoidlH Scherthan
Mar 10, 1997·The Journal of Cell Biology·A V Smith, G S Roeder
Oct 23, 1997·Genes & Development·G S Roeder
Aug 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·D ZenvirthG Simchen
Jan 14, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A ShinoharaD K Bishop
Sep 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y Hiraoka
Jan 9, 1999·The Journal of Biological Chemistry·T de los Santos, N M Hollingsworth
Jan 16, 1999·Current Biology : CB·G S Gordon, A Wright
Feb 3, 1999·Annual Review of Genetics·D Zickler, N Kleckner
Jul 1, 1999·Genes & Development·S M BurgessB M Weiner

❮ Previous
Next ❯

Citations

Feb 16, 2008·Annual Review of Biochemistry·Joseph San FilippoHannah Klein
Jul 4, 2008·Annual Review of Cell and Developmental Biology·Needhi Bhalla, Abby F Dernburg
Jan 24, 2013·PLoS Genetics·Doris Y LuiSean M Burgess
Jan 1, 2012·Scientifica·Tomás Naranjo
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael N ConradMichael E Dresser
Aug 19, 2007·Differentiation; Research in Biological Diversity·Harry Scherthan, Edgar Trelles-Sticken

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.