Analysis of collagen organization in mouse achilles tendon using high-frequency ultrasound imaging

Journal of Biomechanical Engineering
Corinne N RigginLouis J Soslowsky

Abstract

Achilles tendon ruptures are traumatic injuries, and techniques for assessing repair outcomes rely on patient-based measures of pain and function, which do not directly assess tendon healing. Consequently, there is a need for a quantitative, in vivo measure of tendon properties. Therefore, the purpose of this study was to validate ultrasound imaging for evaluating collagen organization in tendons. In this study, we compared our novel, high-frequency ultrasound (HFUS) imaging and analysis method to a standard measure of collagen organization, crossed polarizer (CP) imaging. Eighteen mouse Achilles tendons were harvested and placed into a testing fixture where HFUS and CP imaging could be performed simultaneously in a controlled loading environment. Two experiments were conducted: (1) effect of loading on collagen alignment and (2) effect of an excisional injury on collagen alignment. As expected, it was found that both the HFUS and CP methods could reliably detect an increase in alignment with increasing load, as well as a decrease in alignment with injury. This HFUS method demonstrates that structural measures of collagen organization in tendon can be determined through ultrasound imaging. This experiment also provides a mechan...Continue Reading

References

Oct 1, 1996·Acta Orthopaedica Scandinavica·A MöllerN Westlin
Apr 22, 1999·Injury·S HoushianP Riegels-Nielsen
Oct 2, 2001·British Journal of Sports Medicine·J M RobinsonUNKNOWN Victorian Institute Of Sport Tendon Study Group
Dec 31, 2003·Ultrasound in Medicine & Biology·Tanya GarciaMichael F Insana
Mar 30, 2004·Journal of Biomechanics·Jonathan A GimbelLouis J Soslowsky
Apr 28, 2004·Journal of Biomechanics·T W Tony W LinL J Louis J Soslowsky
Jun 18, 2005·Ultramicroscopy·Samir A ShahBruce K Rubin
Nov 29, 2005·Foot & Ankle International·Amar A SuchakNadr Jomha
Feb 16, 2006·The American Journal of Sports Medicine·Michael J MullaneySteven J Lee
Dec 21, 2007·Seminars in Musculoskeletal Radiology·Viviane KhouryEtienne Cardinal
May 3, 2008·IEEE Transactions on Medical Imaging·G R BashfordK Kulig
Oct 11, 2008·Journal of Applied Physiology·Kyle P Quinn, Beth A Winkelstein
Jun 23, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Spencer P LakeLouis J Soslowsky
Aug 20, 2009·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Karin Grävare SilbernagelJón Karlsson
Dec 1, 2009·Clinics in Sports Medicine·Lisa Chinn, Jay Hertel
Dec 1, 2009·British Journal of Sports Medicine·Håkan AlfredsonLars Ohberg
Jan 6, 2010·Clinical Orthopaedics and Related Research·LeAnn M DourteLouis J Soslowsky
Jun 3, 2010·The Journal of Bone and Joint Surgery. American Volume·Karanjit Singh MangatHari Prem
Nov 26, 2010·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·Amol SaxenaNicola Maffulli
Dec 8, 2010·Micron : the International Research and Review Journal for Microscopy·Benedicto de Campos Vidal, Maria Luiza S Mello
May 1, 2012·Journal of Biomechanics·Sarah Duenwald-KuehlRay Vanderby
Jul 25, 2012·Biophysical Journal·Ivan GusachenkoMarie-Claire Schanne-Klein
Nov 15, 2012·Annals of Biomedical Engineering·Connie S ChamberlainRay Vanderby
Dec 21, 2012·Scandinavian Journal of Medicine & Science in Sports·K KuligG R Bashford
Feb 28, 2013·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Xiang-Mei ChenJin-Rui Wang
Jun 14, 2013·Connective Tissue Research·Marco FranchiMilena Fini

❮ Previous
Next ❯

Citations

Mar 10, 2016·Tissue Engineering. Part C, Methods·Sahitya K DenduluriLewis L Shi
Nov 24, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Fei Fang, Spencer P Lake
Mar 17, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Benjamin R FreedmanLouis J Soslowsky
Sep 28, 2018·Journal of Applied Physiology·Kenton L HaganJosh R Baxter
Mar 26, 2019·Advanced Materials·Benjamin R Freedman, David J Mooney
May 7, 2016·Annals of Biomedical Engineering·A M PardesL J Soslowsky
Apr 3, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Benjamin R FreedmanLouis J Soslowsky
Jun 25, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jessica E AckermanAlayna E Loiselle
Aug 25, 2017·The Journal of the American Academy of Orthopaedic Surgeons·Benjamin R FreedmanLouis Soslowsky
Sep 17, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Corinne N RigginLouis J Soslowsky
Dec 14, 2017·Biomechanics and Modeling in Mechanobiology·Akinjide R Akintunde, Kristin S Miller
Mar 27, 2018·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Todd J Hullfish, Josh R Baxter
Sep 26, 2017·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Takashi KanamotoShuji Horibe
Feb 16, 2017·Frontiers in Oncology·Nirilanto Ramamonjisoa, Ellen Ackerstaff
Nov 3, 2016·Journal of Applied Physiology·George W FryhoferLouis J Soslowsky
Nov 29, 2020·The Journal of Physiology·Sarah E BrashearLucas R Smith
Oct 20, 2020·Journal of Shoulder and Elbow Surgery·Angela TateStephen J Thomas
Jul 30, 2021·Acta Biomaterialia·Patrick M WilliamsonAra Nazarian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.