PMID: 11934212Apr 6, 2002Paper

Analysis of commercial CD34 control samples for quality assurance

Clinical Laboratory
Peter BojkoSiegfried Seeber

Abstract

The amount of CD34+ cells is important to predict the quality of stem cell transplants and ensure safe engraftment after high-dose chemotherapy. Further, daily controls of the patients' peripheral blood CD34+ cell counts are performed to optimize peripheral blood stem cell collection, especially in patients with low CD34+ cell numbers. Therefore, the use of reliable reference samples is mandatory for quality assurance, both in terms of patient safety and reproducibility of results from different laboratories. We report our first experience with CD-Chex CD34, containing stabilized placental cord blood cells in preservative medium with defined concentrations of CD34+ cells. Analysis was performed according to standard operating procedures. Three lots were tested sequentially on a day per day use. The expected CD34+ values were 28.6-, 36.7- and 28.1 microl(-1), respectively, and the mean measured values were 27.4 +/- 2.75 microl(-1) (n = 25, range 21 - 33 microl(-1), coefficient of variation [CV] 10.0%), 29.9 +/- 2.39 microl(-1) (n = 17, range 26 - 35 microl(-1), CV 8.0%), and 27.2 +/- 2.24 micro1(-1) (n = 18, range 24 - 34 microl(-1), CV 8.2%). Serial dilution (1:2 to 1:10) with normal peripheral blood or PBS w/o Ca++/Mg++ gave a...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.