Analysis of commercial kidney stone probiotic supplements

Urology
Melissa L EllisJohn Knight

Abstract

To examine the levels of Oxalobacter formigenes in probiotic supplements marketed by PRO-LAB, Ltd, Toronto, Canada, and capsules of Oxalo purchased from Sanzyme Ltd, Hyderabad, India, and to measure the ability of these preparations to degrade oxalate in vitro. Probiotic supplements and pure cultures of O. formigenes were cultured in a number of media containing oxalate. Optical density at 595 nm (OD595) was used to measure bacterial growth, and ion chromatography was used to measure loss of oxalate in culture media. O. formigenes-specific and degenerate Lactobacillus primers to the oxalate decarboxylase gene (oxc) were used in polymerase chain reaction (PCR). Incubating probiotic supplements in different media did not result in the growth of oxalate-degrading organisms. PCR indicated the absence of organisms harboring the oxc gene. Culture and 16S ribosomal ribonucleic acid gene sequencing indicated the PRO-LAB supplement contained viable Lactococcus lactis subsp. lactis (GenBank accession no. KJ095656.1), whereas Oxalo contained several Bacillus species and Lactobacillus plantarum. The probiotic supplement sold over the Internet by PRO-LAB Ltd and Sanzyme Ltd did not contain identifiable O. formigenes or viable oxalate-degrad...Continue Reading

References

Mar 7, 2008·Journal of the American Society of Nephrology : JASN·David W KaufmanDavid R Cave
Mar 9, 2011·Journal of Endourology·Judith Parsells KellyDavid W Kaufman
Apr 5, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Bernd HoppeDawn Milliner

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Citations

Mar 5, 2016·Journal of Endourology·Thanmaya ReddyDean Assimos
Oct 27, 2016·Archives of Microbiology·Melissa L EllisJohn Knight
Nov 30, 2016·Current Opinion in Urology·Andreas Hiergeist, André Gessner
Jun 19, 2017·Microbial Pathogenesis·Humaira SadafSyed Waqas Hassan
May 17, 2015·Applied and Environmental Microbiology·Xingsheng LiJohn Knight
Sep 25, 2016·Applied and Environmental Microbiology·Xingsheng LiJohn Knight
Jul 23, 2019·The Journal of Infectious Diseases·Amanda M PeBenitoMartin J Blaser
Jan 14, 2020·Journal of Endourology·Kevin KooBrian R Matlaga
Jul 1, 2021·Applied and Environmental Microbiology·Steven L DanielJohn Knight

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