Analysis of complement deposition and processing on Chlamydia trachomatis.

Medical Microbiology and Immunology
Mads LausenSvend Birkelund

Abstract

Chlamydia trachomatis (C. trachomatis) is the leading cause of sexually transmitted bacterial infections worldwide, with over 120 million annual cases. C. trachomatis infections are associated with severe reproductive complications in women such as extrauterine pregnancy and tubal infertility. The infections are often long lasting, associated with immunopathology, and fail to elicit protective immunity which makes recurrent infections common. The immunological mechanisms involved in C. trachomatis infections are only partially understood. Murine infection models suggest that the complement system plays a significant role in both protective immunity and immunopathology during primary Chlamydia infections. However, only limited structural and mechanistic evidence exists on complement-mediated immunity against C. trachomatis. To expand our current knowledge on this topic, we analyzed global complement deposition on C. trachomatis using comprehensive in-depth mass spectrometry-based proteomics. We show that factor B, properdin, and C4b bind to C. trachomatis demonstrating that C. trachomatis-induced complement activation proceeds through at least two activation pathways. Complement activation leads to cleavage and deposition of C3 ...Continue Reading

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Methods Mentioned

BETA
density gradient centrifugation
acetylation
reverse phase chromatography
transmission electron microscopy
FCS
flow cytometry
confocal microscopy
confocal

Software Mentioned

SPSS Statistics
MaxQuant
Perseus

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