Analysis of conjugated and unconjugated bile acids in serum and jejunal fluid of normal subjects

Clinica Chimica Acta; International Journal of Clinical Chemistry
A TangermanE W van der Hoek

Abstract

A reliable method is described for the determination of conjugated and unconjugated bile acids in serum and jejunal fluid. Bile acids are extracted using reverse-phase octadecylsilane bonded silica cartridges and are separated into their unconjugated and conjugated fractions using the lipophilic anion exchanger diethylaminohydroxypropyl Sephadex LH-20 (DEAP-LH-20). The conjugated fraction can be separated into a glycine and a taurine fraction, using the same anion exchanger. The bile acids are measured using a hydroxysteroid dehydrogenase-fluorimetric assay for serum and a hydroxysteroid dehydrogenase-photometric assay for jejunal fluid. The normal fasting serum value of total 3 alpha-hydroxy bile acids amounts to 3.5 +/- 2.8 mumol/l (mean +/- SD, range 1.4-10.8, n = 22). The corresponding unconjugated bile acid fraction amounts to 39.9 +/- 11.2% (range 20.7-64.6%) of total bile acids. The concentration of conjugated bile acids became significantly elevated 30, and 60 min after a standard meal, whereas that of unconjugated bile acids remained unchanged. In jejunal fluid only conjugated bile acids are found, as well in fasting subjects as postprandial, 30 or 60 min after a standard meal.

References

Nov 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·K D SetchellG M Mupthy
Feb 1, 1969·Lancet·B LewisI D Wootton
Aug 31, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·M J Whiting
Jan 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K D Setchell, A Matsui
Jan 1, 1980·Scandinavian Journal of Gastroenterology·A Bremmelgaard, B Almé
Apr 1, 1957·The Journal of Clinical Investigation·D RUDMAN, F E KENDALL

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Citations

Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P W ThimisterJ B Jansen
Apr 1, 1994·Digestive Diseases and Sciences·I P van MunsterF M Nagengast
Jul 28, 2007·Pharmaceutical Research·Greg A KossenaChristopher J H Porter
Nov 13, 2008·Pharmaceutical Research·Dimitrios G FatourosAnette Müllertz
Sep 29, 2001·Advanced Drug Delivery Reviews·C J Porter, W N Charman
Apr 18, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·S YamashitaH Tokuda
Sep 9, 2010·Molecular Pharmaceutics·Deanna M MudieGregory E Amidon
Dec 1, 1993·European Journal of Clinical Investigation·I P van MunsterF M Nagengast
Mar 1, 1993·European Journal of Clinical Investigation·J M SalemansJ B Jansen
Mar 29, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·F MaestrelliP Mura
Mar 12, 2011·International Journal of Pharmaceutics·Anne T LarsenAnette Müllertz
Dec 8, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Selena Di Maio, Rebecca L Carrier
May 24, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Arik Dahan, Amnon Hoffman
Sep 1, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Marloes BerkhoutFokko M Nagengast
Nov 25, 2005·Drug Development and Industrial Pharmacy·Gladys E GraneroGordon L Amidon
Mar 29, 2008·Expert Opinion on Drug Metabolism & Toxicology·Dimitrios G Fatouros, Anette Mullertz
Feb 26, 2004·The Journal of Pharmacy and Pharmacology·Edmund S KostewiczJennifer B Dressman
Jul 29, 2006·The Journal of Pharmacy and Pharmacology·Mariangeles Perez de la Cruz MorenoPatrick Augustijns
May 17, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Zi-qiang LiShu-jun Sun
Aug 1, 1989·European Journal of Clinical Investigation·A MascleeJ H van Tongeren
May 1, 2009·Journal of Colloid and Interface Science·Subhashis ChakrabortySanjay Singh
Mar 3, 2007·Nature Reviews. Drug Discovery·Christopher J H PorterWilliam N Charman
Nov 15, 1997·Journal of Dairy Science·J P Burton, G W Tannock
Mar 17, 2020·Molecular Pharmaceutics·Konstantinos StamatopoulosDavid B Turner
Feb 15, 2001·Journal of Pharmaceutical Sciences·P E Luner, D Vander Kamp
Sep 1, 1994·Applied and Environmental Microbiology·G W TannockM A McConnell

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