PMID: 9543010May 16, 1998Paper

Analysis of constitutive heterochromatin of Aotus (Cebidae, Primates) by restriction enzyme and fluorochrome bands

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
Julio Cesar PieczarkaM S Mattevi

Abstract

The current classification of genus Aotus includes nine species, four of which occur above the Amazon River and five below it. The position of several of these taxa as a valid species has been questioned. Recently, we described the chromosomal constitution of a population in the state of Rondonia, Brazil, whose karyotype typically presented a considerable accumulation of constitutive heterochromatin. To best characterize these heterochromatins, in this work we subjected the metaphases of these animals to banding using AluI, HaeIII, HinfI, RsaI, DdeI, MboI and MspI restriction enzymes and CMA3 and DAPI fluorochromes. The banded metaphases were also submitted to sequential C-banding. RsaI, DdeI and MboI enzymes showed, in all chromosomes, a banding pattern of C type, similar to that obtained using barium hydroxide. This banding was also seen with AluI, HinfI and MspI, but with reduction or elimination of the C-bands in the chromosome pairs 1, 3-7 and 9. MspI also reduced the C-band of pairs 11, 16-21 and 23. HaeIII induced intermediate bands between G and C. Considering the data of the different bands produced, it was possible to characterize at least three distinct types of constitutive heterochromatin in Aotus from Rondonia: (a...Continue Reading

Citations

Apr 13, 2005·Genes & Genetic Systems·Filomena AdegaHenrique Guedes-Pinto
Jun 14, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Ornjira PrakhongcheepAkihiko Koga
Aug 10, 2011·American Journal of Physical Anthropology·Paul L BabbTheodore G Schurr
Aug 16, 2017·Genome Biology and Evolution·Akihiko KogaHirohisa Hirai
Aug 24, 2007·Micron : the International Research and Review Journal for Microscopy·Susana MelesRaquel Chaves

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