PMID: 11318419Apr 25, 2001Paper

Analysis of corticosteroids in equine urine by liquid chromatography-mass spectrometry

Journal of Chromatography. B, Biomedical Sciences and Applications
P W TangT S Wan

Abstract

A liquid chromatography-mass spectrometry (LC-MS) method for the analysis of corticosteroids in equine urine was developed. Corticosteroid conjugates were hydrolysed with beta-glucuronidase; free and enzyme-released corticosteroids were then extracted from the samples with ethyl acetate followed by a base wash. The isolated corticosteroids were detected by LC-MS and confirmed by LC-MS-MS in the positive atmospheric pressure chemical ionisation mode. Twenty-three corticosteroids (comprising hydrocortisone, deoxycorticosterone and 21 synthetic corticosteroids), each at 5 ng/ml in urine, could easily be analysed in 10 min.

Citations

Jul 27, 2001·Journal of Mass Spectrometry : JMS
Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marc CherletPatrick De Backer
Dec 31, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bo A G JönssonPalle Orbaek
Apr 2, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Olga Huetos HidalgoThea B A Reuvers
Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M W J van HoutG J de Jong
Jul 27, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Dongmei ChenZonghui Yuan
Apr 10, 2014·Journal of Analytical Toxicology·Karan AgrawalKaryn Bischoff
May 19, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tiedong GuoSteven J Soldin
May 26, 2004·Clinics in Laboratory Medicine·Jennifer P HolstSteven J Soldin
May 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yan ZhangRu-Qin Yu
Sep 5, 2003·Rapid Communications in Mass Spectrometry : RCM·K Deventer, F T Delbeke
Nov 22, 2007·Journal of Separation Science·Roberto Muñiz-ValenciaRoberto Izquierdo-Hornillos
Oct 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomasz ŚniegockiAndrzej Posyniak
Jan 10, 2021·Equine Veterinary Journal·Michael StuckiKatrin Voelter
Mar 27, 2004·Archives of Pathology & Laboratory Medicine·Tiedong GuoSteven J Soldin

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