PMID: 9159410Jan 1, 1997Paper

Analysis of cytokine producing activity of intestinal intraepithelial T cells from TCR beta-chain and delta-chain mutant mice

Microbiology and Immunology
M KohyamaS Kaminogawa

Abstract

Intestinal intraepithelial T cells (IELs) expressing either gammadelta TCR or alphabeta TCR have been proposed to play an important role in the regulation of intestinal epithelia by producing cytokines that directly influence the adjoining intestinal epithelial cell (IEC) functions. To illuminate this issue, we utilized TCR mutant mice to obtain gammadelta IELs, alphabeta IELs and mixed gammadelta and alphabeta IELs from corresponding alphabeta T-cell-deficient (beta-/-), gammadelta T-cell-deficient (delta-/-) and wild-type (WT) littermate mice. The production of IFN-gamma by these IELs as well as the mRNA for IFN-gamma, TGF-alpha, TGF-beta1, TNF-alpha and TNF-beta in these IELs, in conjunction with the effect of produced cytokines on the expression of class II MHC molecules by the in vitro cell line IEC-6, were investigated. IFN-gamma and TNF-alpha [corrected] specific mRNA were detectable in all freshly isolated gammadelta, alphabeta and WT IELs. In addition to the IFN-gamma and TNF-alpha [corrected] mRNA, alphabeta and WT IELs that had been activated in culture plates coated with anti-CD3 mAb contained mRNA for TGF-beta1 and TNF-beta proteins. In the cultured gammadelta IELs, however, the signals for IFN-gamma and TNF-alpha ...Continue Reading

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Citations

Mar 20, 2003·Immunology Letters·Yuriko HaraHiromasa Ishii
Jul 26, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Wei ZhuFuliang Hu
Apr 20, 2002·Journal of Pediatric Gastroenterology and Nutrition·Frank M RuemmeleMichael J Lentze
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·M KohyamaH Ishikawa
Jun 9, 2005·The Tohoku Journal of Experimental Medicine·Xiumin ZhangRyoichi Nagatomi
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T OidaH Ishikawa

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