Analysis of cytokine receptor messenger RNA expression in human glioblastoma cells and normal astrocytes by reverse-transcription polymerase chain reaction

Journal of Neurosurgery
M TadaN de Tribolet

Abstract

To elucidate which cytokine receptors may be expressed by human glioblastoma and normal astrocytic cells, the presence of messenger ribonucleic acid (RNA) for a number of cytokine receptors was examined in 16 glioblastoma cell lines and adult and fetal astrocytes. A complementary deoxyribonucleic acid copy of total RNA was synthesized and amplified with specific primers using the polymerase chain reaction method. The receptors studied were interleukin (IL)-1 receptor type I (IL-1RI) and type II (IL-1RII), p75 and p55 tumor necrosis factor (TNF) receptors (p75TNFR and p55TNFR), interferon (IFN)-alpha/beta and -gamma receptors (IFN-alpha/beta R and IFN-gamma R), granulocyte-macrophage (GM) colony-stimulating factors receptor alpha subunit (GM-CSFR), G-CSF receptor (G-CSFR), M-CSF receptor (c-fms, M-CSFR), stem cell factor receptor (c-kit, SCFR), IL-6 receptor (IL-6R), and IL-8 receptor (IL-8R). Transcripts for IL-1RI, p55TNFR, IFN-alpha/beta R, and IFN-gamma R were present in all cell lines. The presence of IL-1RII, p75TNFR, GM-CSFR, M-CSFR, SCFR, IL-6R, and IL-8R was identified in 13, eight, seven, eight, 14, three, and one cell lines, respectively. Normal astrocytes were positive for IL-1RI, p75TNFR, p55TNFR, IFN-alpha/beta R, ...Continue Reading

References

Jan 1, 1978·Infection·T KalagerC O Solberg
Dec 1, 1978·Infection and Immunity·I LarssonL Strannegård
Dec 11, 1992·Science·A E KochR M Strieter
May 1, 1992·Immunology Today·L A Tartaglia, D V Goeddel
Dec 1, 1992·Immunology Today·A F LopezM A Vadas
Jun 26, 1992·Brain Research·M SawadaT Marunouchi
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B MotroE Keshet
Sep 1, 1990·Journal of Neuroimmunology·P GalloB Tavolato
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R FukunagaS Nagata
Jul 1, 1991·Journal of Neuroimmunology·E P CowanS Dhib-Jalbut
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·V W YongJ P Antel
Jul 1, 1990·Clinical and Experimental Immunology·M C KuppnerN de Tribolet
Jun 15, 1990·Biochemical and Biophysical Research Communications·K OhnoT Marunouchi
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P LiebermanM L Shin
Dec 1, 1988·Immunology Today·J A Langer, S Pestka
Jun 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·E HelsethG Unsgaard
May 1, 1989·Developmental Biology·L J Regenstreif, J Rossant
Jan 1, 1987·Journal of Neuro-oncology·W K YungM G Rosenblum
Nov 15, 1988·International Journal of Cancer. Journal International Du Cancer·P ZuberN de Tribolet

❮ Previous
Next ❯

Citations

Aug 27, 1999·Journal of Neuroscience Research·Y WangS Fedoroff
Jul 12, 2002·Journal of Neuroscience Research·Jonathan Z PanRonald P Hart
Sep 1, 1993·Journal of Neuro-oncology·M Tada, N de Tribolet
Jun 10, 2011·Neurosurgical Review·Kenichiro IwamiToshihiko Wakabayashi
Sep 16, 2010·Journal of Molecular Neuroscience : MN·Sihem ThabetBesma Bel Hadj Jrad
Jun 12, 2012·Molecular Neurobiology·Soheila Karimi-Abdolrezaee, Rohini Billakanti
Feb 5, 2004·The Breast : Official Journal of the European Society of Mastology·H KnüpferR Preiss
Dec 1, 1996·Journal of the Neurological Sciences·U Müller-LadnerS R Barnum
Mar 1, 2000·Journal of Neuroimmunology·A R DixL A Morford
Jan 7, 1998·Trends in Neurosciences·J L RidetF H Gage
Nov 26, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·U BankS Ansorge
Feb 3, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Juliana Croitoru-LamouryBruce J Brew
Apr 2, 2005·Viral Immunology·Marni N SilvermanAndrew H Miller
Mar 4, 2000·Journal of Neurosurgery·F DiMecoA Olivi
Jan 23, 2010·Neurological Research·Pinar AtukerenBulent Canbaz
May 7, 2014·Annals of Neurology·Frank RichterHans-Georg Schaible
Aug 19, 2007·Cytokine·Athanasios ZisakisAnastasios Kalofoutis
Sep 10, 1999·Cytokine·H PoppenborgJ E Wolff
Apr 1, 1996·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·M Tada, N de Tribolet
Jun 5, 2001·Medical Hypotheses·E C Hurlock
Sep 17, 2011·Molecular Immunology·Laura A FraczekBrian K Martin
Jan 1, 1995·Advances in Neuroimmunology·J M KruegerS Novitsky
Aug 8, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Michelle A Erickson, William A Banks
Jan 27, 2004·Journal of Neuroscience Research·Cornelia Ruedig, Ralf Dringen
Jan 13, 2000·Neuropathology and Applied Neurobiology·J E Merrill, N J Scolding
Jan 1, 1997·Journal of Neuroscience Research·S C Zhang, S Fedoroff

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.