Analysis of cytotoxic effects of chlorhexidine gluconate as antiseptic agent on human blood lymphocytes

Journal of Biochemical and Molecular Toxicology
Ahmad SalimiJalal Pourahmad

Abstract

The aim of this study was to assess the cytotoxicity of chlorhexidine gluconate (CHG) on human blood lymphocytes as a useful ex vivo model for accelerated human toxicity studies. Using biochemical and flow cytometry assessments, we demonstrated that addition of CHG at 1 μM concentration to human blood lymphocytes induced cytotoxicity following 6 h. The CHG-induced cytotoxicity on human blood lymphocytes was associated with intracellular reactive oxygen species generation, mitochondrial membrane potential collapse, lysosomal membrane injury, lipid peroxidation, and depletion of glutathione. According to our results, CHG triggers oxidative stress and organelles damages in lymphocytes which are important cells in defense against foreign agents. Finally our findings suggest that using of antioxidants and mitochondrial/lysosomal protective agents could be of benefit for the people in the exposure with CHG.

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Citations

Jun 7, 2019·Journal of Medical Microbiology·Ali OsmanovDavid W Denning
Aug 12, 2017·Journal of Biochemical and Molecular Toxicology·Sílvio Terra StefanelloFélix Alexandre Antunes Soares
Aug 29, 2020·AAPS PharmSciTech·Pedro Henrique Sette-de-SouzaAna Cláudia Dantas de Medeiros
Dec 2, 2020·American Journal of Infection Control·Timothy L Wiemken, Aaron C Ericsson
Nov 21, 2021·Journal of Materials Science. Materials in Medicine·Sarah J GarnerMichele E Barbour

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