PMID: 2492486Feb 1, 1989Paper

Analysis of cytotoxic T cell responses to intracameral allogeneic tumors

Investigative Ophthalmology & Visual Science
B R Ksander, J W Streilein

Abstract

Injection of P815 mastocytoma tumor cells into the anterior chamber (AC) of BALB/c mice results in progressively growing intraocular tumors, whereas injection of the same dose of tumor cells subcutaneously results in rapid rejection of the tumor. Previous results indicated that AC P815 inoculation results in an unorthodox immune response characterized by suppressed delayed hypersensitivity, elevated antibody levels and priming for cytotoxic T lymphocyte (CTL) activity. Since mice inoculated in the AC with P815 cells display progressively growing intraocular tumors in spite of the fact they are primed for CTL activity, we examined whether the differential tumor growth patterns between AC and SC inoculation are the result of an aberrant and weak cytotoxic response in the former. We analyzed the lytic activity, target specificity and frequency of cytotoxic precursors and effector cells in the spleen following AC and SC inoculation of P815 cells. Our results indicate that both AC and SC routes of P815 inoculation generate splenic CTL effector cells with equivalent lytic activity for P815 tumor target cells. These effectors are highly specific for P815 cells and do not lyse third-party EL-4 targets. Analysis of the frequency of cyto...Continue Reading

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