Analysis of defective protein ubiquitylation associated to adriamycin resistant cells

Cell Cycle
Valérie LangManuel S Rodriguez

Abstract

DNA damage activated by Adriamycin (ADR) promotes ubiquitin-proteasome system-mediated proteolysis by stimulating both the activity of ubiquitylating enzymes and the proteasome. In ADR-resistant breast cancer MCF7 (MCF7ADR) cells, protein ubiquitylation is significantly reduced compared to the parental MCF7 cells. Here, we used tandem ubiquitin-binding entities (TUBEs) to analyze the ubiquitylation pattern observed in MCF7 or MCF7ADR cells. While in MCF7, the level of total ubiquitylation increased up to six-fold in response to ADR, in MCF7ADR cells only a two-fold response was found. To further explore these differences, we looked for cellular factors presenting ubiquitylation defects in MCF7ADR cells. Among them, we found the tumor suppressor p53 and its ubiquitin ligase, Mdm2. We also observed a drastic decrease of proteins known to integrate the TUBE-associated ubiquitin proteome after ADR treatment of MCF7 cells, like histone H2AX, HMGB1 or β-tubulin. Only the proteasome inhibitor MG132, but not the autophagy inhibitor chloroquine partially recovers the levels of total protein ubiquitylation in MCF7ADR cells. p53 ubiquitylation is markedly increased in MCF7ADR cells after proteasome inhibition or a short treatment with the...Continue Reading

References

Mar 15, 1987·Biochemical Pharmacology·B K SinhaC E Myers
Jul 16, 2004·Journal of Proteome Research·Marion L GehrmannYetrib Hathout
Sep 17, 2008·Biochemical Society Transactions·Roland Hjerpe, Manuel S Rodríguez
Nov 10, 2009·Current Hypertension Reports·Mark J Ranek, Xuejun Wang
Jan 19, 2010·The International Journal of Biochemistry & Cell Biology·Roland HjerpeManuel S Rodríguez
Oct 26, 2010·Molecular & Cellular Proteomics : MCP·Yi ShiJun Qin
Dec 20, 2011·Journal of Proteomics·Fernando Lopitz-OtsoaManuel S Rodriguez
Feb 22, 2012·Methods in Molecular Biology·Fabienne AilletManuel S Rodríguez
Mar 17, 2012·BMC Biology·Henning WalczakIvan Dikic
Apr 10, 2012·Annual Review of Biochemistry·Koraljka Husnjak, Ivan Dikic
Nov 28, 2012·Current Pharmaceutical Design·Wendy XolalpaGaël Roué
Mar 8, 2013·Biochimica Et Biophysica Acta·Florian BassermannMichele Pagano
Jul 23, 2013·Biochimica Et Biophysica Acta·Aaron Ciechanover, Ariel Stanhill
Nov 15, 2014·Cells·Zoi ErpapazoglouRosine Haguenauer-Tsapis
Dec 3, 2014·Biochimica Et Biophysica Acta·Marilene DemasiDiego Bonatto
Jan 30, 2015·The Biochemical Journal·Johanna Heideker, Ingrid E Wertz
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yukiko YoshidaKeiji Tanaka
Apr 25, 2015·Trends in Cell Biology·Michael J ClagueSylvie Urbé
May 28, 2016·Experimental Biology and Medicine·Avital Eisenberg-LernerYifat Merbl
Jul 14, 2016·FEBS Letters·Sonia SernaManuel S Rodriguez
Oct 21, 2016·Journal of Ovarian Research·Radosław JanuchowskiMaciej Zabel
Dec 9, 2016·International Journal of Molecular Sciences·Stephen R ArmstrongRoger P Leng
Feb 7, 2017·Cell Cycle·L H GalloD J Donoghue
Feb 22, 2017·International Journal of Molecular Sciences·Sanam Sane, Khosrow Rezvani

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Citations

Mar 2, 2019·Trends in Biochemical Sciences·Michael MatternManuel S Rodriguez
May 4, 2021·Frontiers in Pharmacology·Yuanqi LiuChunfang Zhang

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Methods Mentioned

BETA
affinity capture
immunoprecipitation
flow cytometry

Software Mentioned

ProScanArray® Express
FlowJo
GraphPad Prism®

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