Analysis of degradation of bacterial cell division protein FtsZ by the ATP-dependent zinc-metalloprotease FtsH in vitro

Microbiological Research
Ramanujam SrinivasanParthasarathi Ajitkumar

Abstract

The identity of protease(s), which would degrade bacterial cell division protein FtsZ in vivo, remains unknown. However, we had earlier demonstrated that Escherichia coli metalloprotease FtsH degrades E. coli cell division protein FtsZ in an ATP- and Zn(2+)-dependent manner in vitro. In this study, we examined FtsH protease-mediated degradation of FtsZ in vitro in detail using seven different deletion mutants of FtsZ as the substrates, which lack different extents of specific regions at the N- or C-terminus. FtsH protease assay in vitro on these mutants revealed that FtsH could degrade all the seven deletion mutants irrespective of the deletions or the extent of deletions at the N- or C-terminus. These observations indicated that neither the N-terminus nor the C-terminus was required for the degradation of FtsZ, like already known in the case of the FtsH substrate sigma(32) protein. The recombinant clones expressing full-length FtsZ protein and FtsZ deletion mutant proteins would be useful in investigating the possibility of FtsZ as a potential in vivo substrate for FtsH in ftsH-null cells carrying ftsH suppressor function and ectopically expressed FtsH protease.

References

Sep 17, 1992·Nature·P de BoerL Rothfield
Feb 1, 1991·Research in Microbiology·T OguraS Hiraga
Oct 6, 1995·The Journal of Biological Chemistry·Y AkiyamaK Ito
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·C HermanP Bouloc
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A KiharaK Ito
Feb 10, 1995·Cell·H P Erickson
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·D Bramhill, C M Thompson
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C HermanP Bouloc
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·X MaW Margolin
Mar 1, 1997·Microbiology·Elena LysenkoSimon M Cutting
Jun 1, 1997·Molecular Microbiology·Y ShotlandA B Oppenheim
Aug 8, 1998·Trends in Cell Biology·H P Erickson
Jun 5, 1999·The EMBO Journal·A KiharaK Ito
May 16, 2000·Journal of Bacteriology·Y ShotlandA B Oppenheim
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ogura, A J Wilkinson
Sep 22, 2001·Journal of Bacteriology·T TomoyasuB Bukau
Aug 10, 2002·Journal of Bacteriology·Shinobu ChibaKoreaki Ito
Apr 18, 2003·Journal of Bacteriology·Richard B Weart, Petra Anne Levin
Aug 5, 2003·Journal of Bacteriology·Jesse Stricker, Harold P Erickson

❮ Previous
Next ❯

Citations

Oct 17, 2012·The Journal of Antimicrobial Chemotherapy·Sandhya A MaratheDipshikha Chakravortty
Sep 20, 2011·Biochimica Et Biophysica Acta·Sina LangklotzFranz Narberhaus
Jan 1, 2010·Acta Biochimica Et Biophysica Sinica·Prabuddha GuptaParthasarathi Ajitkumar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.